ANESTHETIC ACTION ON THE ATP-SENSITIVE POTASSIUM CHANNEL

描述：(摘自申请者的摘要)本研究的目标 是为了确定吸入性呼吸之间相互作用的细胞机制 麻醉药与心脏和心脏的ATP敏感性钾通道(KATP) 研究这种相互作用在缺血条件下是如何受到影响的。 围手术期心肌缺血可导致术后不良反应 心脏和非心脏手术，但麻醉作用的机制 在缺血期，我们还不是很清楚。其中一个 心脏在缺血期间发生的电生理变化是 激活KATP通道。这些通道由新陈代谢控制 心脏的状态，并可能作为心脏保护机制在 缺血症。因此，麻醉作用的一个潜在目标是 缺血是KATP通道。到目前为止，对药物影响的研究 吸入性麻醉药在细胞水平对心脏KATP通道的作用 是有限的。这份提案中描述的实验将描述 KATP通道与吸入麻醉药的相互作用 膜片钳方法。具体目标是：1)确定 吸入麻醉药对KATP影响的机制 2)测定麻醉作用对KATP的影响 在缺血条件下的通道，以及3)表征麻醉剂如何 改变钾通道开放剂激活KATP的能力 频道。初步结果显示，吸入麻醉剂对 对KATP通道的不同影响。因此，对细胞的解释 KATP通道上麻醉作用的机制有FAR 达到临床意义。根据使用的麻醉剂的类型， 它可能增强或减少心脏潜在的保护机制。 心内KATP通道。

DESCRIPTION: (Adapted from the applicant's abstract) The goal of this study is to identify the cellular mechanism of interaction between inhalational anesthetics and the ATP-sensitive potassium channel (KATP) in heart and to investigate how this interaction is affected during ischemic conditions. Perioperative myocardial ischemia can result in deleterioius effects after both cardiac and noncardiac surgery, but the mechanisms of anesthetic action during the ischemic period are not well understood. One of the electro-physiological changes that occur in the heart during ischemia is activation of KATP channels. These channels are gated by the metabolic state of the heart and may serve as a cardioprotective mechanism during ischemia. Consequently, a potential target of anesthetic action during ischemia is the KATP channel. To date, studies of the effects of inhalational anesthetics on the KATP channel in heart at the cellular level are limited. The experiments described in this proposal will characterize the interaction between the KATP channel and inhalational anesthetics using the patch clamp methodology. The specific objectives are 1) to determine the mechanism of the effects of inhalational anesthetics on the KATP channels; 2) to determine the effects of anesthetic action on the KATP channels during ischemic conditions, and 3) to characterize how anesthetics alter the ability of potassium channel openers to activate the KATP channels. Preliminary results show that inhalational anesthetics exert differential effects on the KATP channel. Thus, elucidation of the cellular mechanism responsible for anesthetic action on the KATP channel has far reaching clinical significance. Depending on the type of anesthetic used, it may enhance or reduce the potentially cardioprotective mechanism of the KATP channel in heart.