REDUCTIVE CLEAVAGE METHOD FOR GLYCAN STRUCTURAL ANALYSIS

描述：聚糖是在所有生物中发现的聚合碳水化合物。 东西 它们在身体中具有根本的重要性，因为它们 参与细胞间通讯和其他细胞识别 过程，它们在传染病中具有根本的重要性 由于它们作为抗原决定簇存在于细菌、真菌和 病毒 在分子水平上确定这些分子的结构 并建立这些分子中重要的结构基序 识别过程是该领域的中心主题之一， 但是，不幸的是，朝着这些目标的进展一直是 由于缺乏真正通用的结构分析方法， 少量的材料。 本提案的主要目标之一是， 是发展电化学还原裂解作为一种手段， 表征聚糖。 指导该方法的发展将是其 应用于合成制备的低聚糖的分析 文库，糖蛋白和糖脂衍生的寡糖，以及 荚膜多糖和脂多糖来源于几个 牙龈卟啉单胞菌菌株，牙龈卟啉单胞菌的主要致病因子之一， 牙周病 本建议的另一个主要目标是 开发一种还原裂解方法的变体，至少 1000-折叠更敏感。 所提出的方法将利用 还原分析法测定电子捕获GLC检测器灵敏度 裂解片段作为它们的三氟丙基醚/三氟丙酰基酯 衍生物. 指导这种方法的发展也将是其 应用于糖蛋白和糖脂衍生的寡糖。

DESCRIPTION: Glycans are polymeric carbohydrates found in all living things. They are of fundamental importance within the body because of their involvement in cell-cell communication and other cellular recognition processes and they are of fundamental importance in infectious disease because of their presence as antigenic determinants in bacteria, fungi, and viruses. Determining the structures of these molecules at a molecular level and establishing the structural motifs important in these molecular recognition processes is one of the central themes in the field of glycobiology but, unfortunately, progress toward these goals has been hampered by the lack of a truly general method for structural analysis using small amounts of material. One of the major goals of the present proposal is to develop electrochemical reductive cleavage as a means to fully characterize glycans. Guiding the development of the method will be its application to the analysis of synthetically prepared oligosaccharide libraries, glycoprotein- and glycolipid-derived oligosaccharides, and the capsular polysaccharides and lipopolysaccharides derived from several strains of Porphyromonas gingivalis, one of the major causative agents of periodontal disease. The other major goal of the present proposal is to develop a variant of the Reductive-Cleavage Method that is at least 1000-fold more sensitive. The proposed method will take advantage of the sensitivity of an electron capture GLC detector by analyzing reductive cleavage fragments as their trifluoropropyl ether/trifluoropropionyl ester derivatives. Guiding the development of this method will also be its application to glycoprotein- and glycolipid-derived oligosaccharides.