Flow Cytometry and Confocal Microscopy Shared Resource

流式细胞术和共聚焦显微镜共享资源（CYT-SR）：摘要 CYT-SR为佛罗里达大学健康癌症中心（UFHCC）成员提供具有成本效益的， 先进的流式细胞术和现代共聚焦显微镜专业知识，仪器和服务，以支持他们的 研究活动。CYT-SR是由UFHCC支持的机构管理的共享资源（SR） 通过与UF生物技术研究跨学科中心（ICBR）的伙伴关系。它位于同一地点 在癌症和遗传学研究中心和麦克奈特脑研究所，提供方便的访问， UFHCC成员。CYT-SR拥有4名员工，拥有超过22年的综合经验，包括Kellish， 科学总监，免疫学家，在流动分析的各个方面拥有超过7年的经验。关键 CYT-SR中可用的技术包括多参数流式细胞术、细胞分选、活细胞成像， 共聚焦显微镜，基于表面生物标志物的癌症和免疫细胞表型，组织和 通过共聚焦显微镜观察细胞形态，以及小颗粒和外泌体分析。在最近的COVID-19期间 大流行，CYT-SR被确定为一个重要的校园资源，并保持开放和访问， 所有用户仪器预订、设备使用时间和技术人员辅助活动由 UFHCC并通过CrossLab实验室管理系统收费。自2016年以来，CYT-SR 由78个独特的UFHCC用户（43个具有同行评审资金）使用，占所有用户的24%。 设施。UFHCC成员的CYT-SR使用量从2016年的900小时增加到2021年的4，800小时，增加了5. 3倍。 CYT-SR支持了180多篇出版物，其中24%的IF >10。自2019年CCSG提交以来， 来自UFHCC成员实验室的484名学生，博士后研究员和技术人员接受了CYT培训， SR仪器。肿瘤发生机制与肿瘤治疗和宿主反应成员 代表了UFHCC的大多数用户。CYT-SR在加强成员研究方面的有效性 生产力通过年度调查、CYT-SR科学咨询小组的反馈进行评估， 监督委员会的建议。由于对CYT-SR资源的需求不断增加， UFHCC支持购买新的索尼SP 6800光谱分析仪（2018），Cytoflex LX 21 Becton Dickinson Symphony A3（2020）。为了支持首次在人体内进行的RNA疫苗临床试验， UFHCC支持为CYT-SR招聘一名博士级免疫学家，以进行基于流程的免疫学研究。 免疫分型CYT-SR的未来增长与UFHCC战略计划Momentum保持一致 2027年，和用户满意度调查，并包括升级，如收购BD FACSYMPHONY S6 细胞分选仪，提高超高分辨率显微镜和单细胞分析的能力和服务， 与BQS-SR合作，扩大生物统计学应用于细胞计数的高级培训 分析.

FLOW CYTOMETRY AND CONFOCAL MICROSCOPY SHARED RESOURCE (CYT-SR): ABSTRACT CYT-SR provides University of Florida Health Cancer Center (UFHCC) members with cost-effective, state-of- the-art flow cytometry and modern confocal microscopy expertise, instrumentation, and services to support their research activities. The CYT-SR is an institutionally-managed shared resource (SR) that is supported by UFHCC through a partnership with the UF Interdisciplinary Center for Biotechnology Research (ICBR). It is co-located within the Cancer & Genetics Research Complex and McKnight Brain Institute, providing convenient access for UFHCC members. The CYT-SR has 4 staff with over 22 years combined experience including Kellish, the Scientific Director, an immunologist with more than 7 years of experience in all aspects of flow analysis. Key technologies available in the CYT-SR include multi-parameter flow cytometry, cell sorting, live cell imaging via confocal microscopy, surface biomarker-based cancer and immune cell phenotyping, assessment of tissue and cell morphology by confocal microscopy, and small particle and exosome analysis. During the recent COVID-19 pandemic, the CYT-SR was identified as an essential campus resource and remained open and accessible to all users. Instrument reservations, equipment usage time, and technician-assisted activities are tracked by the UFHCC and charged back via the CrossLab laboratory management system. Since 2016, the CYT-SR was utilized by 78 unique UFHCC users (43 with peer-reviewed funding) accounting for ~24% of all users of the facility. CYT-SR use by UFHCC members increased 5.3-fold from 900 hours in 2016 to 4,800 hours in 2021. The CYT-SR supported more than 180 publications, with 24% having IF >10. Since the 2019 CCSG submission, 484 students, postdoctoral fellows, and technical staff from UFHCC member laboratories were trained on CYT- SR instruments. Mechanisms of Oncogenesis and the Cancer Therapeutics and Host Response members represent the majority of UFHCC users. The effectiveness of the CYT-SR in enhancing member research productivity is assessed by annual surveys, feedback from the CYT-SR Scientific Advisory Group, and recommendations from the SR Oversight Committee. Due to the rising demand for CYT-SR resources by members, the UFHCC supported the purchase of a new Sony SP6800 Spectral Analyzer (2018), Cytoflex LX21 (2020), and a Becton Dickinson SYMPHONY A3 (2020). To support first-in-human RNA vaccine clinical trials, UFHCC supported the recruitment of a PhD-level immunologist to the CYT-SR to perform flow-based immunophenotyping. Future growth of the CYT-SR are in alignment with the UFHCC strategic plan, Momentum 2027, and user satisfaction surveys, and include upgrades such as an acquisition of a BD FACSYMPHONY S6 cell sorter, increasing capabilities and services in super high-resolution microscopy and single cell analysis, and in collaboration with BQS-SR, expansion of advanced training in the application of biostatistics to cytometric analysis.