T CELL RESPONSE TO AA IN MICE

再生障碍性贫血与局限性青少年牙周炎密切相关， 患者牙周袋中发现的多种细菌中的一种 患有成人牙周炎。已有研究表明，无论是细胞介导的还是 体液免疫反应在牙周病中起重要作用。的确有 还有一种怀疑，即夸大的免疫反应可能是 对于所见的大部分破坏，即骨丢失、软组织破坏。 还没有显示的是在分子水平上到底是什么相互作用 在T细胞和再生障碍性贫血之间。我们建议利用杂交瘤技术 问一问AA和TO的主要T细胞抗原表位是什么 确定小鼠是否存在受限的TCR反应。 由于口腔感染，如牙周病，允许 病原菌附着并定植在牙周袋中而不 直接暴露在宿主的免疫系统中，我们会问是否 当小鼠被感染和免疫时，曲目是不同的。刻画 对AA的免疫反应，我们将分析TCR序列。如果有几个 我们培育的杂交瘤具有相同的Vβ但不同的T细胞受体 连接，存在超抗原性反应的可能性。另一个 可能的情况是，免疫优势表位可能在 该连接加上V区暗示了一个主要的抗原表位。 通过将抗原特异性与TCR连接序列相关联， 我们可以阐明AA对T细胞诱导的调节作用 感染。对AA的免疫反应的这些特征都是 疫苗设计中的重要考虑因素。

Aa has been strongly associated with localized juvenile periodontitis and is one of many species of bacteria found in the gingival pocket of patients with adult periodontitis. It has been shown that both cell mediated and humoral immune responses are important in periodontal diseases. There is also a suspicion that an exaggerated immune response could be responsible for much of the destruction seen, i.e., bone loss, soft tissue destruction. What has not been shown is exactly what interaction at the molecular level is seen between T cells and Aa. We propose to utilize hybridoma technology to ask what are the predominant T cell antigenic epitopes on Aa and to determine whether there is a restricted TcR response in mice. Since an oral infection such as periodontal disease allows for the pathogenic bacteria to adhere and colonize the gingival pocket without direct exposure to the host's immune system, we will ask whether the repertoire differs when mice are infected versus immunized. To characterize the immune response to Aa, we will analyze TcR sequences. If several of the hybridomas we raise have T cell receptors with the same V beta but different junctions, the possibility of a superantigenic response exists. Another possibility is that an immunodominant epitope could exhibit conservation in the junction plus the V regions suggesting a predominant antigenic epitope. By correlating the antigenic specificity with the TcR junctional sequences, we can elucidate the regulation of T cell induction in response to Aa infection. These characteristics of the immune response to Aa are all important considerations in vaccine design.