PLASMA PROTEINS: MARKERS OF CHEMICAL EXPOSURE

我们的长期目标是确定化学物质暴露在 生物体液和开发程序(S)，通过该程序可以建立这种标记(S) 以职业性接触化学品和 因此可以对可能产生的不利影响提出建议。 在拟议的研究中，我们计划研究化学物质暴露的影响。 对血浆蛋白质的生物活性，浓度， 和共价键修饰。这些变化将使用生物和 免疫分析、电泳法和层析技术。Covelent 修饰将通过肽图进一步表征， 氨基酸组成和序列分析。的结构。 经修饰的氨基酸(S)将通过光谱技术和 经独立合成进一步证实。苯、三氯乙烷和 环氧乙烷将被用作模型化合物。 尽管拟议的项目仅限于大鼠和体外研究 人类血浆，这些实验可以扩展到职业监测 暴露在这些或其他化学品中的人群。两者之间的相关性 接触者血浆蛋白变化与病史的关系 个人最终可以被用来进行医学监测。

Our long term goal is to identify the markers of chemical exposure in biological fluids and develop procedure(s) by which such marker(s) can be measured in the population who are exposed occupationally to chemicals and thus can be advised for possible adversed effects. In the proposed research we plan to study the effects of chemical exposure on plasma proteins in terms of their biological activity, concentration, and covalent modification. These changes will be measured using bio- and immunoassays, electrophoretic and chromatographic techniques. Covelent modification will be further characterized by peptide mapping, compositional and sequential amino acid analysis. The structure of the modified amino acid(s) will be determined by spectral techniques and further confirmed by independent synthesis. Benzene, ethylenechloride and ethylene oxide will be used as model compounds. Although the proposed project is limited to rats and in vitro studies with human plasma, these experiments could be extended to monitor occupationally exposed population to these or other chemicals. Correlation between the changes in plasma proteins and the medical histories of the exposed individuals can eventually be used to medical surveillance.