AEQUORIN MEASUREMENT OF CYTOSOLIC CA IN PAROTID CELLS

腮腺腺泡细胞胞浆钙(CaI)的测定 了解腮腺囊性纤维化的原因 囊性纤维化患者的细胞钙含量增加60%，并对 神经兴奋剂是不正常的。对钙敏感的水通道蛋白似乎是 测量CAI的最佳指标，因为它没有不良反应 效果(即缓冲CAI，改变CAI转运体的动力学， 自淬火等)Quin 2和Fura 2。因为Aequorin必须是散装的 然而，加载到细胞中，需要确认加载 手术不会改变正常的腮腺细胞功能。这笔赠款 应用是确定腮腺细胞是否能承受负荷 程序。这将通过比较cGMP和 Aequorin改变了由β-肾上腺素能或 胆碱能刺激。最后，我们将确定是否有变化 在钙-45内流、钙-45外流、环磷酸腺苷和环磷酸腺苷的诱发性增强中 Aequorin的存在引起的环状GMP。

Measurement of cytosolic calcium (Cai) in the parotid acinar cells is very important in understanding the cause of cystic fibrosis since the parotid cells of cystic fibrosis patients have 60% more calcium and respond to neurostimulants abnormally. The calcium sensitive aequorin seems to be the best indicator for measurement of Cai because it does not have the untoward effect (i.e. buffering Cai, altering the kinetics of Cai transporters, self-quenching etc.) of Quin 2 and Fura 2. Since aequorin must be bulk loaded into the cell, however, it is necessary to confirm that the loading procedure does not alter the normal parotid cell function. This grant application is to determine whether parotid cells can withstand the loading procedure. This will be done by comparing the cGMP, and ATP of the aequorin has altered the amount of amylase released by beta adrenergic or cholinergic stimulation. Finally, we shall determine if there are changes in the evoked enhancement of Ca-45 influx, Ca-45 efflux, cyclic AMP and cyclic GMP due to the presence of aequorin.