Carbohydrate Microarray Profiling of Glycan-Binding Antibodies and Lectins

癌细胞在发病期间经历碳水化合物表达的急剧变化， 疾病的进展。因此，人们对理解为什么 这些变化的发生，它们如何导致疾病，并利用这些变化， 临床应用。碳水化合物结合抗体和凝集素在其中起着关键作用。 研究有几个原因。首先，抗体和凝集素被广泛用于检测和 监测碳水化合物表达的变化。在这种情况下，它们用于基本的 研究，诊断和治疗药物。全面了解他们的 特异性对于正确使用和解释结果至关重要。直到最近， 没有现成的资料。随着碳水化合物微阵列技术的出现， 我们现在可以快速分析这些蛋白质与各种碳水化合物的结合 抗原此外，该阵列可用于开发新的抗体和凝集素， 监测新的碳水化合物抗原我们已经研制出了几种抗体 碳水化合物抗原正在使用抗体来研究肿瘤中的碳水化合物表达。 第二，许多天然碳水化合物结合蛋白如半乳糖凝集素参与了蛋白质的合成。 肿瘤发生和转移。因此，人们对开发 配体以调节其活性。我们的碳水化合物微阵列非常适合评估 结合并鉴定天然凝集素的配体。

Cancer cells undergo dramatic changes in carbohydrate expression during the onset and progression of disease. As a result, there has been considerable interest in understanding why these changes occur, how they contribute to the disease, and exploiting these changes for clinical use. Carbohydrate binding antibodies and lectins play a critical role in this research for several reasons. First, antibodies and lectins are used extensively to detect and monitor changes in carbohydrate expression. In this capacity, they are used for basic research, as diagnostics, and as therapeutic agents. A comprehensive understanding of their specificity is critical for proper use and interpretation of results. Until recently, this information was not readily available. With the advent of carbohydrate microarray technology, we can now rapidly analyze binding of these proteins to a wide variety of carbohydrate antigens. In addition, the array can be used to develop new antibodies and lectins for monitoring new carbohydrate antigens. We have been developing antibodies to several carbohydrate antigens are using the antibodies to study carbohydrate expression in tumors. Second, many natural carbohydrate binding proteins such as galectins are involved in tumorigenesis and metastasis. As a result, there has been considerable interest in developing ligands to modulate their activity. Our carbohydrate microarray is well-suited to evaluate binding and identify ligands for natural lectins.