Role of Novel MicroRNAs in the PVT-1 Locus

覆盖PVT 1基因座的基因组序列的计算分析和实验分析 验证鉴定了7个miRNAs。事实上，所有的miRNA前体转录物都是 在晚期B细胞（包括PCT和vBL细胞系）中的表达水平高于 未成熟的B细胞提示可能在淋巴发育和/或淋巴瘤中的作用。在 此外，慢病毒载体介导的miR-15 PVT 1前体（人和 小鼠）在小鼠前B细胞系中增加了Myc的表达，这表明一个阶段 靶点的特异性下调必然对Myc表达有一定的间接影响。高 hsa-miR-15 PVT 1前体的表达水平也见于几种上皮癌中， MYC/PVT 1共扩增的细胞系表明这些细胞可能具有更广泛的作用， miRNAs与肿瘤发生

Computational analysis of the genomic sequence covering the PVT1 locus and experimental verification identified seven miRNAs. Virtually all the miRNA precursor transcripts are expressed at higher levels in late-stage B cells (including PCT and vBL cell lines) compared to immature B cells suggesting possible roles in lymphoid development and/or lymphoma. In addition, Lentiviral vector-mediated overexpression of the miR-15PVT1 precursor (human and mouse) in a mouse pre-B cell line increased expression of Myc, suggesting that a stage specific down-regulation of a target(s) must have some indirect effect on Myc expression. High levels of expression of the hsa-miR-15PVT1 precursor is also seen in several epithelial cancer cell lines with MYC/PVT1 co-amplification suggesting a potentially broader role for these miRNAs in tumorigenesis.