Pathology and Laboratory Support Core

病理学和实验室支持核心是支持各种研究的有效核心 方案项目通过提供常规和高级技术准备，包括形态 细胞、组织和器官制剂的分析。核心提供诊断光学显微镜评估， 组织切片的荧光显微镜和免疫组织化学解释 小鼠前列腺组织和前列腺癌骨转移。此外，技术先进的专家 在包括定量RT-PCR和组织原位杂交在内的广泛方法中得到了协助 可用。利用动物身体的项目调查人员也可以使用先进的 技术，如透射电子显微镜和图像分析/形态计量学。提供设施 包括组织学(特殊染色、冰冻切片、石蜡和塑料包埋切片)和 免疫组织化学。 核心的一个关键优势是，它能够统一描述和传播共享集合 组织学标准。研究肿瘤-骨-基质相互作用的关键是分离的能力 相对纯净的肿瘤和周围间质样本。与我们的计划项目特别相关 是在原发肿瘤和转移的骨标本中这样做的能力。病理学的核心 是否会获得新鲜的前列腺组织，包括：原发肿瘤、肿瘤相关间质、良性上皮、 直接形态学下临床标本的转移骨标本和与肿瘤无关的间质 使用激光捕获显微镜进行分子分析的可视化；b)作为形态核心， 为临床标本和前列腺癌动物模型提供前列腺癌诊断专业知识 癌症，保持冰冻切片和光镜设施，组织化学，免疫组织化学和 原位杂交显微镜支持；以及c)提供充分的具有良好特征的、存档的病理 用于筛选具有潜在机械重要性的候选分子的样本，细胞培养的验证 以及动物模型实验数据和新生物标志物的发现，包括高通量分析- 组织微阵列。 相关性(请参阅说明)： 核心C将继续提供诊断和分子分析细胞和组织样本从 人类和动物。这将允许更全面的质量测试，以及更多种类的高级 方法：研究方法。程序标准化和质量受控，导致更大的技术一致性和 允许在项目之间进行直接比较。调查人员将继续随时可以进入 先进的技术，如透射电子显微镜和图像分析。

The Pathology and Laboratory Support Core is an effective nucleus that supports the various studies of the program project by providing routine and advanced technical preparations, including morphological analysis of cells, tissue and organ preparations. The core provides diagnostic light microscopic evaluation, epifluorescent microscopy of histological sections and immunohistochemical interpretation of human and murine prostatic tissue and prostate cancer bone metastases. In addition, technologically advanced expert assistance in a broad range of methods, including quantitative RT-PCR and tissue in-situ hybridization are available. Program project investigators utilizing animal necropsies also have access to sophisticated techniques, such as transmission electron microscopy and image analysis/morphometry. Facilities available include histology (special stains, frozen, paraffin- and plastic-embedded sections) and immunohistochemistry. A key advantage of the core is that it enables unified characterization and dissemination of a shared set of histologic standards. Key to the study of the tumor-bone-stromal interaction is the ability to isolate relatively pure samples of the tumor and the surrounding stroma. Particularly relevant to our program project is the ability to do so in the primary tumor as well as in bone specimens with metastasis.The pathology core will a) procure fresh prostatic tissue, including: primary tumor, tumor-related stroma, benign epithelium, metastatic bone specimens, and stroma unrelated to tumor from clinical specimens under direct morphologic visualization using laser capture microscopy for molecular analyses; b) serve as the morphology core, providing prostate cancer diagnostic expertise for both clinical specimens and animal models of prostate cancer, maintain frozen section and light microscopic facilities, histochemistry, immunohistochemistry and in situ hybridization microscopic support; and c) provide ample well-characterized, archived pathologic samples for screening of candidate molecules of potential mechanistic importance, validation of cell culture and animal model experimental data anddiscovery of novel biomarkers, including high throughput analysis - tissue microarray. RELEVANCE (See instructions): Core C will continue to provide both diagnostic and molecular analysis of cell and tissue samples from human and animals. This will allow for more comprehensive quality testing with wider variety of advanced methods. Procedures are standardized and quality-controlled, leading to greater technical uniformity and allowing for direct comparisons between projects. Investigators will continue to have ready access to sophisticated techniques such as transmission electron microscopy and image analysis.