Core B - Technical

答:介绍。怀孕的绵羊将继续被用于这些研究，因为它们很容易获得，相对便宜（与灵长类动物相比），胎儿足够大，可以研究足够数量的几种组织（大脑、子宫、肺动脉、肾上腺等），我们和其他人已经积累了相当多的关于这个物种的生理数据。基本上，本提案中所有的单个项目都将使用怀孕或未怀孕的绵羊，这些绵羊已经适应了高海拔、长期低氧血症或常氧控制。在献祭时，这些动物的组织将被分配给本提案的几个研究人员和项目，以及其他几个研究人员。因此，本技术核心包括用于这些研究的动物。此外，它还包括大多数这些实验所需的几项主要设备的费用。实验动物和设备技术核心将被中心所有成员用于几乎所有共同项目。这样的技术核心优化了实验室、实验本身以及数据采集和分析的有效运作。实验动物采购和实验室的基本特征详细说明如下。在对我们的实验室、设备和实验动物设施进行总体描述之后，技术核心将从几个方面进行考虑：实验动物、白山研究站、LLU动物设施、实验室核心设施和预算。

A. Introduction. Pregnant sheep will continue to be used for these studies, as they are readily available, are relatively inexpensive (as compared with primates), the fetuses are large enough so that adequate amounts of the several tissues can be studied (cerebral, uterine, and pulmonary arteries, adrenal glands, ef cetera), and we and others have amassed a considerable body of physiologic data on this species. Essentially all of the individual projects in this proposal will use pregnant or nonpregnant sheep which have been acclimatized to high altitude, long-term hypoxemia, or normoxic controls. At the time of sacrifice, tissues from these animals will be partitioned among the several investigators and projects of this proposal, as well as several other investigators. Thus, this Technical Core includes the animals for these studies. In addition, it includes the expense for several major items of equipment required for most of these experiments. The experimental animal and equipment Technical Core will be utilized for essentially every project in common by all members of the Center. Such a Technical Core optimizes the efficient operation of the laboratories, the experiments per se, and the data acquisition and analysis. The essential features of the experimental animal procurement and laboratory are detailed below. Following a general description of our laboratories, equipment, and laboratory animal facilities, the Technical Core will be considered from several aspects: Experimental Animals, White Mountain Research Station, LLU Animal Facilities, Laboratory Core Facilities, and Budget. Drs. Longo and Ducsay will devote 10% of their time to administer the Technical Core. The principal investigators will continue to share major items of equipment including: imaging systems, ultracentrifuges, centrifuges, radioactivity counters, recorders, blood-gas analyzers, analyzers for Western immunoblot, realtime PCR, confocal microscopy, freezers, and other items. In addition, we will continue to share the experimental animals, and surgical operating theater. This fiscally efficient arrangement, together with the scientific collaboration among the investigators js one of the reasons that broad based research support is sought.