Mouse and cMyBP-C Protein Production Core

该核心将被安置在辛辛那提儿童医院医学中心的分子 心血管生物学。虽然在空间上与计划项目赠款团队的成员分开。博士 罗宾斯已经与他们合作多年，并提供目前的计划项目赠款 研究人员和核心与小鼠和蛋白质片段。 核心将是关键的研究提出的所有计划项目补助金的组成部分，因为它将是 负责所有转基因小鼠的设计、生产、群体维护和运输， 由项目资助的研究人员和核心B提出。核心也会产生不同的 体外和分离系统研究所需的蛋白、蛋白片段和抗体。因此它 是计划项目资助基金会不可或缺的一部分。 核心的职责是： 设计和构建所有构建体，用于基于细菌或Sacu/o的MyBP-C蛋白和蛋白片段生产。 培养和纯化足够数量的所有蛋白质片段，以满足研究人员的需求 设计，建造和生成所有在计划项目资助中提出的转基因小鼠。 在屏障设施中保持足够数量的所有线路，以便根据需要将其分发给调查人员以及要求他们的其他科学家。 作为cMyBP-C特异性抗体的制备、维护和质量控制的中心信息交换所 保持上述所有物品的足够库存，并安排方便的运输给各个研究者或核心领导人。

This Core will be housed at the Cincinnati Children's Hospital Medical Center within the Division of Molecular Cardiovascular Biology. Although spatially separated from members of the Program Project Grant team. Dr. Robbins has been collaborating with them for years and supplies the current Program Project Grant investigators and Cores with both mice and protein fragments. The Core will be critical for the studies proposed in all of the Program Project Grant's Component's as it will be responsible for the design, production, colony maintenance and shipment of ail transgenic mice that are proposed by the Program Project Grant's investigators and Core B. The Core will also produce the different proteins, protein fragments and antibodies needed for the in vitro and isolated systems studies. As such, it forms an integral part of the Program Project Grant's foundation. The Core's responsibilities are: ¿ Design and build all constructs for bacterial- or Sacu/o-based MyBP-C protein and protein fragment production. ¿ Grow and purify all protein fragments in sufficient quantities to satisfy the investigators' needs ¿ Design, build and generate all transgenic mice that are proposed in the Program Project Grant. ¿ Maintain sufficient numbers of all lines in barrier facilities so that they can be distributed as needed to the investigators as well as to other scientists who request them. ¿ Serve as a central clearing house for the preparation, maintenance and quality control of the cMyBP-C specific antibodies ¿ Maintain an adequate inventory of all of the above and arrange for convenient shipping to the individual investigators or Core Leaders.