THE ROLES OF PROHIBITINS (PHBS) IN 3T3-L1 ADIPOCYTE DIFFERENTIATION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 A.具体目标 本提案的目的是确定在3 T3-L1脂肪细胞分化过程中，白藜芦醇苷的功能意义。我们的初步数据表明，这两个阿比丁（PHB），包括PHB 1和PHB 2（雌激素受体活性的阻遏物，REA），在3 T3-L1前脂肪细胞的脂肪细胞分化过程中显着增加。此外，3 T3-L1脂肪细胞分化受损后，同时敲低任一PHBs蛋白的siRNA-PHB 1或siRNA-REA。因此，我们的第一个目标是确定PHB 1和PHB 2是否是促进小鼠3 T3-L1脂肪细胞分化的关键因素。我们的第二个目的是研究PHB 1和PHB 2在3 T3-L1细胞脂肪形成过程中的信号通路。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A. Specific Aims The goal of this proposal is to determine the functional significance of prohibitins during 3T3-L1 adipocyte differentiation. Our preliminary data showed that both of these prohibitins (PHBs), including PHB1 and PHB2 (repressor of estrogen receptor activity, REA), were significantly increased during the adipocyte differentiation of 3T3-L1 preadipocytes. Moreover, 3T3-L1 adipocyte differentiation was impaired after the simultaneous knockdown of either PHBs protein by siRNA-PHB1 or siRNA-REA. So, our first aim is to determine whether PHB1 and PHB2 are critical factors in promoting mouse 3T3-L1 adipocyte differentiation. Our second aim is to investigate the signaling pathway of PHB1 and PHB2 during 3T3-L1 cell adipogenesis.