ANIMAL CORE

动物中心将提供规划，执行和解释在人类心血管疾病的大型动物模型中进行的实验的专业知识。动物中心的资源允许人道和有效地利用猪进行生存手术、导管插入术和血流动力学评估。这些资源通过各种其他转化研究项目顺利运作，包括心血管设备和诊断工具的开发;我们地区的大型动物实验在许多情况下直接导致了人体临床试验。对于本提案中的项目，将通过我们小组建立和操作的技术进行猪缺血性心肌功能障碍模型的准备和仪器化。将在模拟和平行计划的临床研究的条件下并使用器械将骨髓源性间充质干细胞和心脏干细胞体内递送至猪心脏。动物中心的负责人也是项目1和3临床试验的主要参与者，在猪模型中使用更新的细胞递送技术将有利于临床研究中人类受试者的安全性，因为动物中心获得的经验直接转移到临床研究中。

The Animal Core will provide expertise in planning, performing, and interpreting experiments performed in large animal models of human cardiovascular disease. The resources of the Animal Core allow humane and efficient utilization of swine for survival surgery, catheterization procedures, and hemodynamic assessments. These resources have operated smoothly through a variety of other translational research programs including the development of cardiovascular devices and diagnostic tools; large animal experiments in our area have led in many cases directly to human clinical trials. For the projects in this proposal, preparation and instrumentation of swine to model ischemic myocardial dysfunction will be done by techniques established and operating in our group. Delivery of bone marrow derived mesenchymal stem cells and of cardiac stem cells to swine hearts in vivo will be done under conditions and using devices which mimic and parallel planned clinical studies. The director of the Animal Core is also a key participant in the clinical trials in Projects 1 and 3, and the use of newer cell delivery techniques in the swine model will benefit the safety of human subjects in the clinical studies, as the experience gained in the Animal Core transfers directly to the clinical studies.