EXTRACELLULAR MATRIX ON B-CASEIN GENE EXPRESS IN NORMAL MAMMARY EPITHELIAL CELL

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 高等生物的细胞发育部分是由细胞核和周围环境之间的通讯控制的。 细胞外环境，而越来越多的证据表明，细胞结构的变化是S的遗传物质 对这一通信的监管至关重要。细胞外环境无法正常沟通 有核是肿瘤发展的标志之一。我们已经开发了一个模型系统，在其中正常 乳腺上皮细胞对生理相关的富含层粘连蛋白的三维基底膜(IRBM)作出反应 通过经历功能分化。这项测试将被用来确定细胞外环境如何 以调节特定基因表达的方式影响细胞遗传物质的结构和组织 基因。为了识别这些变化，我们将使用生化和显微技术的组合。 表征细胞外环境对乳腺细胞发育的作用将有助于更好地 了解正常的乳房细胞发育和基因表达，并可能对乳房有影响 肿瘤发生和疾病干预。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cell development in higher organisms is controlled in part by communication between the nucleus and the surrounding extracellular environment, and accumulating evidence shows that changes in the structure of a cell''''s genetic material are central to the regulation of this communication. The inability of the extracellular environment to communicate properly with the nucleus is one of the hallmarks of tumor development. We have developed a model system in which normal mammary epithelial cells respond to a physiologically relevant three-dimensional, laminin-rich basement membrane (IrBM) by undergoing a functional differentiation. This assay will be used to determine how the extracellular environment influences the structure and organization of a cell's genetic material in a manner that regulates the expression of specific genes. To identify these changes, we will employ a combination of biochemical and microscopy techniques. Characterizing the role of the extracellular environment on mammary cell development will contribute towards a better understanding of normal breast cell development and gene expression and may also have implications for breast tumorigenesis and disease intervention.