Development of Molecular Pharmacodynamic Assays for Targeted Therapies

在人类中，AktlPKB家族包括三个高度同源的成员，称为Akt 1、Akt 2和Akt 3。Akt是生长因子、细胞因子和其它细胞刺激物下游的细胞信号传导中的中心节点。Akt的factory激活是多种复杂疾病（包括2型糖尿病和癌症）的病理生理学特性的基础。组成性激活的Akt促进细胞存活以及对药物治疗的抗性。化疗和/或放疗。已经开发了几种靶向Akt的抗癌药物。在许多人类癌症中观察到高水平的磷酸化（活化）Akt，并且在人类和小鼠组织样品中Akt状态的测定在生物医学研究、癌症诊断和预后中具有巨大的潜在应用。 作为一家抗体开发公司，我们致力于生产试剂和抗体，以制造高灵敏度的药效学ELISA测定法，用于测量总Akt，其亚型和磷酸化Akt的水平，以响应mTORIAkt抑制剂和调节剂。重组Akts将用作对照标准品。将抗体试剂配制为夹心ELISA，以从组织或细胞培养物中捕获总Akt。高度标准化的Akt ELISA试剂盒的可用性将有助于调查用于治疗许多疾病的Akt调节试剂的有效性的作用。

In humans, the AktlPKB family comprises three highly homologous members known as Akt1, Akt2, and Akt3. Akt is a central node in cell signaling downstream of growth factors, cytokines, and other cellular stimUli. Faulty activation of Akt underlies the pathophysiological properties of a variety of complex diseases, including type-2 diabetes and cancer. Constitutively activated Akt promotes cellular survival as well as resistance to treatment with . chemotherapy and/or radiation therapy. Several anticancer drugs targeting Akt have been developed. High levels of phosphorylated (activated) Akt are observed in numerous human cancers and the determination of Akt status in human and mouse tissue samples has great potential application in biomedical investigation, cancer diagnosis and prognosis. As an antibody development company, we are committed to produce reagents and antibodies to manufacture highly sensitive pharmacodynamic ELISA assays for measureing both total Akt, its isoforms and level of phosphorylated Akt in response to mTORIAkt inhibitors and modulators. Recombinant Akts will be used as the control standard. The antibody reagents will be formulated as sandwich ELISA to capture total Akt from tissue or cell culture. The availability of a highly standardized Akt ELISA kit will assist the investigation into the role of effectiveness of Akt modulating reagents used to treat a number of diseases.