Cell Culture and Genetic Engineering Core

细胞培养和遗传工程核心有两个组成部分：一、细胞培养组成部分： 该部分的目的是为肝脏研究中心的研究人员提供：(I)分离的肝脏 持续高质量的细胞，(Iii)特征良好的冰冻细胞系，(Iv)专门化 最佳细胞生长所需的培养液、激素和补充剂以及基质成分；以及(Iv) 用于分离和培养来自肝脏的各种细胞类型的设备和说明 和二.基因工程部分：(I)目标是协助肝脏中心的调查人员 发展重组病毒载体，(基于肿瘤逆转录病毒、慢病毒、腺病毒和 杆状病毒)用于其特定研究，以及(2)维护显微镜设备并协助 在基因或其他操作后对培养细胞进行常规评估。要添加的服务 2008年包括(I)培养人类胚胎干细胞并将其提供给肝脏中心的研究人员 用于分化为肝细胞；(Ii)提供从切除的肝脏分离的原代人肝细胞 不用于移植的切段和获取的肝脏；以及(Iii)协助调查人员 移植到动物模型后对肝细胞进行基因标记以进行鉴定。

The Cell Culture and Genetic Engineering Core has two components: I. Cell culture component: Objectives of this component are to provide Liver Research Center Investigators with: (i) isolated liver cells of consistently high quality, (iii) well-characterized frozen stocks of cell lines, (iv) specialized culture media, hormones and supplements, and matrix components for optimal cell growth; and (iv) equipment and instructions for use in isolation and culturing of various cell types derived from the liver and II. Genetic Engineering component: (i) The objectives are to assist Liver Center Investigators in developing recombinant viral vectors, (based on oncoretroviruses, lentiviruses, adenoviruses and baculoviruses) for use in their specific research, and (ii) Maintain equipment for microscopy and assist in routine evaluation of cultured cells following genetic or other manipulations. Services to be added in 2008 include (i) culturing human embryonic stem cells and providing these to Liver Center Investigators for differentiation into liver cells; (ii) providing primary human liver cells isolated from resected liver segments and procured livers that are not used for transplantation; and (iii) assisting Investigators in genetically marking liver cells for identification after transplantation into animal models.