Physiological and Molecular Imaging

这个核心的目标是为各种不同的生物和分子成像提供“真正的FIME”支持 本计划中的试验性应用。核心将提供设备和专业知识，以 以及在两个不同的水平上对细胞和分子相互作用的定量分析：细胞-细胞和细胞分子 体外相互作用，以及体内细胞与细胞之间的相互作用在血管壁的背景下。试管苗 这一核心的组件将使PI能够获得活细胞成像功能，以及计算机辅助成像 分析未标记的细胞，或用荧光标记的抗体标记的细胞，或转导表达的细胞 GFP标记的蛋白质。该部件称为活细胞荧光显微镜设备。用于活体内 研究表明，在血管生物学卓越中心建立的活体显微镜(IVM)设施将 为麻醉动物的微循环提供最先进的实时成像能力。 因此，通过这个核心，计划调查人员将利用对单个人来说过于昂贵的资源 购买和维护实验室。重要的是，核心将提供专业知识和技术援助，以 提高个别项目的生产力，以及提高私人投资机构的能力，以完成 每个项目的实验目标。

The goal of this Core is to provide "real fime" physiological and molecular imaging support for various experimental applicafions in this Program. The Core will provide equipment and expertise for the qualitative and quantitafive analysis of cellular and molecular interactions at two different levels: cell-cell and cellmolecular interactions in vitro, and cell-cell interactions in vivo in the context of the vessel wall. The in vitro component of this Core will give Pis access to live cell imaging capabilifies, and computer assisted image analysis, of unlabeled cells, or cells labeled with fluorescently tagged antibodies or transduced to express GFP-tagged protein. This component is referred to as the Live cell fluorescence Microscopv facility. For in vivo studies, the established Intravital Microscopv (IVM) facility in The Center for Excellence in Vascular Biology will make available state-of the art capabilities for real-time imaging ofthe microcirculation in anesthetized animals. Thus through this Core, Program investigators will leverage resources that would be too expensive for single laboratories to purchase and maintain. Importanfiy, the Core will provide expertise and technical assistance to increase the productivity of individual projects as well as increase the capabilities of the Pis to accomplish the experimental aims of each project.