Enzyme-based approach to probe the specificity of heparanase and its inhibitor

描述（由申请人提供）：细胞外基质的各种成分的降解，包括硫酸肝素蛋白聚糖，是癌症侵袭和转移过程中的关键步骤。肝素酶是一种降解HS的内切p- d -糖苷酶，由于其促转移、促炎症和促血管生成的活性，已成为抗癌治疗的一个有希望的靶点。因此，我们寻求利用一种新的酶法来探测肝素酶及其抑制剂的特异性。

DESCRIPTION (provided by applicant): The degradation of various constituents of the extracellular matrix including, heparan sulfate proteoglycans, represents a critical step in the process of cancer invasion and metastasis. Heparanase, an endo-p-D- glycosidase which degrades HS, has emerged as a promising target for the development of anti-cancer treatment because of its pro-metastatic, pro-inflammatory, and pro-angiogenic activity. Therefore we sought to utilize a novel enzymatic approach to probe the specificity of heparanase and its inhibitor.