INVESTIGATION OF THE [4FE-4S] CLUSTER IN APS REDUCTASE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 最近的晶体学表征表明，独特的[4Fe-4S]簇的硫酸盐还原酶，APS还原酶的活性位点的协调。然而，结构（2.7 <$）的适度分辨率不允许详细分析簇的几何形状，其中包括两个不变的相邻半胱氨酸残基的配位。XAS将用于提供APS还原酶中独特的[4Fe-4S]簇的详细局部结构信息。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recent crystallographic characterization suggests unique [4Fe-4S] cluster coordination in the active site of the sulfatereducing enzyme, APS reductase. However, the modest resolution of the structure (2.7 ¿) does not permit detailed analysis of cluster geometry, which includes coordination by two invariant adjacent cysteine residues. XAS will be used to provide detailed local structure information for the unique [4Fe-4S] cluster in APS reductase.