TISSUE DONATION FOR UNDERSTANDING BRAIN TUMOR BIOLOGY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 脑肿瘤组织收集计划的目的是提供人类脑肿瘤组织样本，这些样本对于验证胶质瘤侵袭的许多新治疗靶点至关重要。将使用该材料进行的试验包括测定活化状态的生化试验和测定这些靶标表达水平的定量RT-PCR（逆转录酶聚合酶链反应）和免疫组织化学。部分样品还将用于建立原代脑肿瘤细胞培养物，用于功能测定和动物模型的建立。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of the brain tumor tissue collection program is to provide human brain tumor tissue samples that are essential for the validation of a number of novel therapeutic targets for glioma invasion. Assays that will be performed using this material include biochemical assays to determine the activation status and quantitative RT-PCR (reverse transcriptase polymerase chain reaction) and immunohistochemistry to determine the expression levels of these targets. Part of the samples also will be used to establish primary brain tumor cell cultures for functional assays and establishment of animal models.