ShEEP Request for Nanostring nCounter Spring Profiler

摘要 在此应用中，我们请求购买Nanostring nCounter Sprint Profiler仪器。的 nCounter Sprint Profiler是一款用户友好、占用空间小、中等通量的仪器，旨在检测 并在单个样品中定量多达800种不同的RNA或DNA序列。这项技术允许表达 在单个细胞或整个生物体中进行分析，并且被设计为在单个qPCR之间弥合差距。 反应和全球技术，如表达阵列或全基因组测序。这项技术 代表了基因组研究的一个重大进展，通过允许检测和定量数百个 以比传统方法低得多的成本和时间靶序列。以下研究项目将 由升级系统支持： 1)Robert基思，医学博士，主要研究者：BLR&D优异评审奖“肺用PPAR γ激动剂 癌症化学预防” 2)霍华德李，医学博士，首席研究员：BLR&D职业发展奖“PPARgamma的作用 肺癌的进展和转移” 3)Nicholas Walter，MD，首席研究员：CSR&D职业发展奖“血液表达 概况作为结核病反应的生物标志物” 4)Subbiah Pugazhenthi，博士，主要研究者：BLR&D Merit Review Award“CREB的机制 阿尔茨海默病大脑的失调” 5)林恩希斯利，博士，主要研究者：BLR&D优秀评论奖“FGFR 1致癌基因驱动因子 头颈癌的发病机制” 6)丽莎布伦纳，博士，共同首席研究员：CSR&D优秀评论奖“弓形虫， 犬尿氨酸途径和退伍军人的自杀行为” 7)Edward Chan，医学博士，Merit Review和丹佛研究所首席研究员

Abstract In this application we request the purchase of a Nanostring nCounter Sprint Profiler instrument. The nCounter Sprint Profiler is a user friendly, small-footprint, medium throughput instrument designed to detect and quantify up to 800 different RNA or DNA sequences in a single sample. This technology allows expression analysis in a single cell or an entire organism and is designed to bridge the gap between single qPCR reactions and global technologies like expression arrays or genome wide sequencing. This technology represents a major advance in genomic research, by allowing detection and quantification of hundreds of target sequences at much lower cost and time than traditional methods. The following research projects will be supported by the upgrades system: 1) Robert Keith, MD, Principal Investigator: BLR&D Merit Review Award “PPAR gamma Agonists for Lung Cancer Chemoprevention” 2) Howard Li, MD, Principal Investigator: BLR&D Career Development Award “The Role of PPARgamma in Lung Cancer Progression and Metastasis” 3) Nicholas Walter, MD, Principal Investigator: CSR&D Career Development Award “Blood Expression Profiles as a Biomarker for Responses to Tuberculosis” 4) Subbiah Pugazhenthi, PhD, Principal Investigator: BLR&D Merit Review Award “Mechanism of CREB dysregulation in Alzheimer brain” 5) Lynn Heasley, PhD, Principal Investigator: BLR&D Merit Review Award “An FGFR1 oncogene driver pathway in head and neck cancer” 6) Lisa Brenner, PhD, Co-Principal Investigator: CSR&D Merit Review Award “Toxoplasma gondi, the kynurenine pathway, and suicidal behavior in veterans” 7) Edward Chan, MD, Principal Investigator, Merit Review and Denver Research Institute