New methods for the modification of peptides and proteins with the Re(CO)3 moiety

 描述(申请人提供)：随着研究人员发现有机金属化合物作为蛋白质动力学的探针、可能的药物和显像剂以及有机转化的绿色催化剂的新用途，生物分子的有机金属功能化变得越来越重要。尽管有这样的兴趣，但几乎没有合成方法来合成具有有机金属基团的蛋白质和多肽，大多数化学方法都集中在与氨基酸侧链基团如组氨酸的直接结合上。这项建议将提出一个计划，以扩大用于制造有机金属蛋白和多肽结合物的反应化学，重点放在Re(CO)3化合物的化学上。该提案中概述的战略的三个目标是：开发合成方法来制造Re修饰的多肽，用Re修饰蛋白质的新技术，以及以Re为中心的合成氨基酸和多肽：这项工作是一个博士授予机构和一个本科生机构之间的合作项目。这项工作将在两个地点进行：阿克伦大学Christopher J.Ziegler教授的实验室和圣十字学院Richard Herrick教授的实验室。除了支持研究计划外，这笔赠款的资金将用于培训本科生和研究生合成无机和生物化学方法，并将改善阿克伦大学和圣十字学院的研究环境。

 DESCRIPTION (provided by applicant): The organometallic functionalization of biological molecules has increased in importance as researchers have found new uses for organometallic compounds as probes for protein dynamics, as possible pharmaceuticals and imaging agents, and as green catalysts for organic transformations. In spite of this interest, there are few synthetic methods for the functionalization of proteins and peptides with organometallic groups, with most of the chemistry focusing on direct binding to amino acid side chain groups like histidine. This proposal will present a plan to expand the reaction chemistry used to make organometallic protein and peptide conjugates, with a focus on the chemistry of Re(CO)3 compounds. The three aims to the strategy outlined in the proposal are: Developing synthetic methodologies for making rhenium-modified peptides, new techniques for modifying proteins with rhenium, and rhenium-centered synthetic amino acids and peptides: This work is a collaborative project between a Ph.D. granting institution and an undergraduate institution. This work will be carried out in two locations: in the laboratories of Prof. Christopher J. Ziegler at te University of Akron, as well as in the laboratories of Prof. Richard Herrick at the College of the Holy Cross. In addition to supporting the research program, funds from this grant will be used to train undergraduate and graduate students in synthetic inorganic and biochemical methods, and will enhance the research environment at the University of Akron and the College of the Holy Cross.