Development of cabbage seedpod weevil resistant canola

加拿大油菜产业每年为我国经济贡献至少10亿美元，而卷心菜籽荚象鼻虫每年造成的损失高达4000万美元，本提案的目标是向加拿大的公共和商业油菜育种者提供一整套遗传控制的、环境可持续的卷心菜籽荚象鼻虫抗性。可验证的将包括1）来源于高品质卡诺拉种质中的杂草亲戚的象鼻虫抗性，和2）与快速渐渗抗性相关的分子标记，基于密集的田间筛选和昆虫行为与鉴定的化学引诱剂和驱避剂植物生物谱直接相关。先前鉴定的抗性在由白色芥菜产生的加倍单倍体中的可用性白芥（Sinapis alba）X油菜（Brassica napus）BC 2代使我们的研究独特之处在于我们已经具有许多对象甲（Ceutorhynchus obstrictus（Marsham）（鞘翅目：象甲科））表达强抗性（R）或易感性（S）的等基因系。这些R和S同工酶为我们提供了一个强有力的起点，以产生分子标记，也使我们能够清楚地探索，比较和确定负责的生化化合物，非法特定的昆虫取食和产卵行为。了解吸引/排斥象鼻虫在其生命周期的各个阶段（进食，产卵等）的化合物将首先使我们能够产生基于S. alba抗性，以及第二，改变生物途径以通过单循环的小孢子诱变和体外选择直接使用优良卡诺拉品系产生新的抗性卡诺拉基因型。

The Canadian canola industry contributes at least one billion dollars annually to the ecomomy of our country, and the cabbage seedpod weevil is capable of causing up to $40,000,000 in annual losses.The goal of this proposal is to deliver a complete package of genetically controlled, environmentally sustainable cabbage seedpod weevil resistance to public and commercial canola breeders in Canada. Deliverables will include 1) weevil resistance sourced from a weedy relative in high quality canola germplasm, and 2) molecular markers associated with resistance for rapid introgression, based on intense field screening and insect behaviour directly correlated with identified chemoattractant and repellent plant bioprofiles.Availability of previously identified resistance in doubled haploids generated from a white mustard (Sinapis alba) x canola (Brassica napus) BC2 generation makes our research unique in that we already have a number of isogenic lines that express either strong resistance (R) or susceptibility (S) to the weevil (Ceutorhynchus obstrictus (Marsham) (Coleoptera: Curculionidae). These R and S isolines provide a powerful starting point for us to generate molecular markers, and also enable us to clearly explore, compare and identify the responsible biochemical compounds that illicit specific insect feeding and oviposition behaviours. Understanding the compounds that attract/repel weevils at various stages of their life cycle (feeding, ovipositing etc.) will, firstly enable us to generate resistant canola germplasm based on S. alba resistance and secondly, to alter the biopathways to generate novel resistant canola genotypes through a single cycle of microspore mutagenesis and in vitro selection, using elite canola lines directly.