Hormonal regulation of mineral metabolism in fishes and invertebrates

目前的建议旨在继续我们的长期计划的研究如何钙和磷酸盐的稳态调节鱼类和水生水蛭的肽激素，斯钙素-1（STC-1）。鳟鱼鳃中富含叶绿素的氯细胞具有高水平的STC-1受体。我们将探讨STC-1靶向氯细胞线粒体作为STC-1抑制鳃钙转运的作用机制的一部分的可能性。 目标二。鳟鱼卵巢中的早期卵母细胞产生STC-1的变体形式，该变体形式似乎在自分泌或内分泌环中被靶向回到这些卵母细胞。我们试图鉴定激素靶向的亚细胞区室，并纯化STC-1的变体形式用于化学分析和生物测定。水蛭有STC相关的蛋白质和受体，调节皮肤对钙的吸收。我们将研究钙等因素对这一转运过程的调节。我们还将确定水蛭的钙吸收是否随时间（周）而循环，就像鱼类一样。我们还将纯化两种不同形式的水蛭STC，然后在结构上进行表征，并在生物学研究中对其进行测试。

The current proposal seeks to continue our long term program of studies on how calcium and phosphate homeostasis are regulated in fish and aquatic leeches by the peptide hormone, stanniocalcin-1 (STC-1).Objective I. The mitochondria-rich, chloride cells in trout gill have high levels of STC-1 receptors. We will explore the possibility that STC-1 is targeted to chloride cell mitochondria as part of the mechanism of action by which STC-1 inhibits gill calcium transport.  Objective II. Early oocytes in trout ovary produce a variant form of STC-1 that appears to be targeted back to these oocytes in an autocrine or intracrine loop. We seek to identify the sub-cellular compartment targeted by the hormone and to purify the  variant form of STC-1 for chemical analysis and bioassay.Objective #3. Leeches have STC-related proteins and receptors that regulate calcium uptake by the skin. We will study the regulation of this transport process by factors such as calcium. We will also determine if calcium uptake in leeches cyclical over time (weeks), as it is in fishes. We will also purify two different leech forms of STC, characterize then structurally and test them in biological studies.