Upgrade for delta vision imaging system

生物体的形态和功能是由精确控制的细胞分裂、分化和死亡决定的。许多实验室正在进行的研究表明，初级纤毛，直到最近才被认为是大多数细胞的退化细胞器，是决定单个细胞命运的信号整合的场所。Quarmby关注的是理解纤毛如何调节细胞分裂，Leroux实验室研究纤毛的发生和纤毛的组成。这两个小组的发现都影响了我们对被称为纤毛病的人类疾病家族的理解。德尔塔视觉成像站（DV）是2002年由CFI新机遇计划和BC省知识发展基金提供的大笔赠款购买的，是SFU纤毛研究的基石。DV是一款高端集成的epi-荧光显微镜系统，包括一个独特的照明系统，它提供了一个具有快速变化激发和发射滤光片能力的均匀光源，一个可以在三维空间中以非常高和可重复的运动进行控制的舞台，一个具有出色光学器件的显微镜，以及一个集成工作站，控制光源，舞台，光学和通过冷却电荷耦合器件相机捕获的图像。失焦光通过反褶积去除。通过这个系统，我们获得了令人惊叹的图像，并取得了重大发现。经过五年的过度使用，这台设备已经老化了。有些零件磨损，有些零件过时了。DV对于我们继续进行高质量的细胞生物学研究是必不可少的，升级是绝对需要的，以保持它的工作秩序。

The form and function of organisms is determined by precisely controlled cell division, differentiation and death. Ongoing research in numerous laboratories is revealing that the primary cilium, until recently considered a vestigial organelle for most cells, is the site of the integration of signals that determine the fate of individual cells. The Quarmby is concerned with understanding how the cilium regulates cell division and the Leroux lab studies ciliogenesis and the composition of cilia. Both groups have made discoveries that impact our understanding of a family of human diseases known as ciliopathies. The DeltaVision Imaging station (DV), which was purchased in 2002 with very large grants from the CFI New Opportunities program and from the BC Knowledge Development fund, is a cornerstone of ciliary research at SFU. The DV is a high-end integrated epi-fluorescence microscopy system that includes a unique illumination system which provides an even light source with capability of rapid changes in excitation and emission filters, a stage that can be controlled with very high and repeatable motion in three dimensions, a microscope with superb optics, and an integrated workstation that controls the light source, the stage, the optics and image capture by a cooled charge-coupled device camera. Out-of-focus light is removed by deconvolution. With this system we have achieved stunning images and made significant discoveries. After five years of extremely heavy use, the equipment is showing its age. Some parts are worn and others are obsolete. The DV is essential for our continued production of high quality cell biological research and the upgrade is absolutely required to keep it in working order.