High resolution multisensor respirometer

有了这笔赠款，我们将购买Oroboros Oxygraph 2K，测量溶解氧和离子。它将使我们能够准确地测量细胞和线粒体的耗氧率。每个动物细胞都含有线粒体，线粒体消耗氧气，同时将摄入的食物转化为可用的能量。线粒体耗氧方式的改变对许多自然和病理现象都很重要。例如，在冬眠中，哺乳动物的耗氧率下降了90%，因为它的体温福尔斯降到接近冰点。肝脏线粒体代谢在冬眠中减少了70%，使用Oxygraph，我们将研究这种情况是如何发生的。这些发现可能会改善创伤性损伤的治疗和用于移植的器官的活力。

With this grant we will purchase an Oroboros Oxygraph 2K that measures dissolved oxygen and ions. It will allow us to accurately measure rates of oxygen consumption of cells and mitochondria. Every animal cell contains mitochondria which consume oxygen while converting the products of ingested food into usable energy. Changes in the way mitochondria consume oxygen are important to many natural and pathological phenomena. For example, in hibernation a mammal's rate of oxygen consumption decreases by up to 90% as its body temperature falls close to freezing. Liver mitochondrial metabolism is decreased by 70% in hibernation and, using the Oxygraph, we will investigate how this occurs. These findings may improve treatment for traumatic injury and the viability of organs used for transplant.