Analytical equipment for detecting the concentration and chemical speciation of trace metals and reactive oxygen species in natural waters

海洋学界正在开展一项重大的国际项目（GEOTRACES http://www.geotraces.org/），研究微量元素和同位素的海洋生物地球化学循环，并阐明其分布中的海洋学信息。该计划包括对所有主要海洋盆地的详细水柱部分进行采样，并在战略位置区域进行过程和时间序列研究，并将承担沉重的分析负担。这个项目的规模在化学海洋学领域是前所未有的，并有望在理解过去和现在的海洋过程及其在地球气候系统变化中的作用方面提供一个巨大的飞跃。例如，一些微量元素，如钴、铜、铁和锌，是必需的微量营养素。更好地了解控制这些可能限制生物的微量营养素的分布和种类形成的因素，将为了解它们在调节海洋生态系统的结构和生产力方面的作用、这些生态系统隔离二氧化碳的效率及其对气候变化的敏感性提供关键信息。该提案要求获得资金来组装流动注射分析(FIA)/序列注射分析（SIA）系统的组件，该系统设计用于化学发光，比色和荧光法检测海水中的生物活性痕量金属和活性氧。这些分析方法已被证明具有成本效益、高通量、精确的技术，可用于近实时测定海水中溶解的痕量金属，可以满足像GEOTRACES这样的分析密集型程序的要求。

The oceanographic community is undertaking a major international program (GEOTRACES http://www.geotraces.org/) to study the marine biogeochemical cycling of trace elements and isotopes and to elucidate the oceanographic information embedded in their distribution. The program consists of sampling detailed water column sections in all major ocean basins, complemented by process and time-series studies in strategically located regions and will carry with it a heavy analytical burden. The scale of this program is unprecedented in the field of chemical oceanography and promises to provide a quantum leap forward in the understanding of ocean processes past and present and their role in Earth's changing climate system. For instance, some trace elements, such as Co, Cu, Fe, and Zn, are essential micronutrients. A better understanding of the factors controlling the distribution and speciation of these potentially biolimiting micronutrients will provide critical information for understanding their role in regulating the structure and productivity of marine ecosystems, the efficiency of these ecosystems at sequestering carbon dioxide and their sensitivity to climate change. This proposal requests funds to acquire components to assemble a flow injection analysis (FIA)/sequence injection analysis (SIA) system designed for the chemiluminescent, colorimetric and fluorometric detection of bioactive trace metals and reactive oxygen species in seawater. These analytical approaches have proven to be cost effective, high throughput, precise techniques for near real-time determinations of dissolved trace metals in seawater that can meet the requirements of analytically intense programs like GEOTRACES.