Chemiluminescence and fluorescence imaging system for biochemical, molecular and cellular biology applications

Wilfrid Laurier University （WLU）生物化学系和细胞生物学、生物化学和分子生物学领域的研究人员将利用化学发光学和荧光成像系统：研究细胞内和细胞外原生动物对鱼类细胞系的影响；表征髓磷脂中各种膜微结构域；并确定蛋白质的靶向和进入叶绿体的机制。所要求的成像系统将支持所有三个共同申请人的研究项目（除了其他当前的，以及可能未来的两个部门的研究人员），并有助于培养本科生和研究生。成像系统包括一个高分辨率的超冷CCD相机，具有各种激发源和发射滤波器。该系统将允许可见光、紫外、荧光和化学发光应用于1D和2D凝胶、Western、Southern和Northern blots、菌落计数、生物/组织成像、宏阵列、TLC板和微量滴度板。因此，该系统将在支持和加强广泛的研究项目方面发挥重要作用，这样做将有助于支持两个部门新建立的研究生项目，从而促进WLU理学院不断增长的研究强度。

Researchers in the Departments of Biology and Chemistry at Wilfrid Laurier University (WLU) working in the fields of cell biology, biochemistry, and molecular biology will utilize the chemiluminscence and fluorescence imaging system to: study the effects of intracellular and extracellular protozoans on fish cell lines; characterize the various membrane microdomains in myelin; and determine the mechanism of protein targeting and import into chloroplasts. The requested imaging system will support the research programs of all three co-applicants (in addition to other current, and likely future researchers in both departments), and contribute to the training of undergraduate and graduate students. The imaging system includes a high-resolution ultra-cooled CCD camera with various excitation sources and emission filters. The system will permit visible, UV, fluorescence, and chemiluminescence applications for 1D and 2D gels, Western, Southern, and Northern blots, colony counting, organism/ tissue imaging, macroarrays, TLC plates, and microtiter plates. Therefore, the system will play a significant role in supporting and enhancing a broad range of research programs, and in so doing will help support newly established graduate programs in both departments, and thereby contribute to the growing research intensity within the Faculty of Science at WLU.