A microscopical examination of curdlan production by an Agrobacterium sp.

一株农杆菌产生凝胶酶的显微观察。我们将研究从农杆菌细胞表面分泌的不溶性多糖Curdlan，一种(1，3)-β-葡聚糖，以及单个多糖链组装成微纤维的过程。使用最先进的时间推移和电子显微镜技术，我们将比较野生型可聚糖生产细胞的图像和在可得聚糖生产过程中受损的突变细胞的图像。这些产出将是关于可德兰生产机制的信息，这些信息将补充我们的分子、生物和生物化学研究得出的信息。这将对了解细菌多糖的生产具有普遍意义，并可能在提高可德聚糖生产方面产生商业化结果。

A microscopical examination of curdlan production by an Agrobacterium sp. We will investigate the secretion of the insoluble polysaccharide curdlan, a (1,3)-beta-glucan, from the surfaces of Agrobacterium cells and the assembly of the individual polysaccharide chains into microfibrils. Using state-of-the-art techniques in time lapse and electron microscopy we will compare the images of wild type curdlan-producing cells with those of mutants impaired in the production of curdlan. The outputs will be information on the mechanics of curdlan production that will complement that emerging from our molecular biological and biochemical studies. These will have implications for understanding bacterial polysaccharide production in general and may have a commercial outcome in enhanced curdlan production.