Gas Phase Reactivity of Charged Peptide and DNA Radicals: Fundamentals and Applications

带电肽和DNA自由基的气相反应性：基础和应用。来自“生命分子”，蛋白质和DNA的自由基，既发挥有益的作用（如酶催化），也发挥有害的作用（如与疾病相关的蛋白质和DNA损伤）。已经发现两种电喷雾电离质谱方法产生相关模型系统（例如肽和核碱基）的带电自由基。这些物质的气相化学在很大程度上是一个未知的领域！我们将研究这些系统的基本化学（单分子和双分子反应），并建立在一些令人兴奋的初步结果，这些结果表明潜在的应用（例如作为蛋白质组学工具来测序和区分肽中的亮氨酸和异亮氨酸残基）。

Gas Phase Reactivity of Charged Peptide and DNA Radicals: Fundamentals and Applications. Radicals derived from the "molecules of life", proteins and DNA, play both beneficial (e.g. enzyme catalysis) and deleterious roles (e.g. protein and DNA damage associated with disease). Two electrospray ionisation mass spectrometry approaches have been discovered to generate charged radicals of related models systems (e.g. peptides and nucleobases). The gas phase chemistry of these species is a largely unchartered area! We will examine the fundamental chemistry (unimolecular and bimolecular reactions) of these systems and build upon some exciting preliminary results which suggest potential applications (e.g. as a proteomics tool to sequence and distinguish between leucine and isoleucine residues in peptides).