Biosynthesis of nonribosomal peptide toxins in cyanobacteria: A functional characterisation of microcystin synthetase

蓝藻中非核糖体多肽毒素的生物合成：微囊藻毒素合成酶的功能特征。微囊藻毒素是与蓝藻水华相关的蓝藻产生的一种强有力的毒素和肿瘤促进剂。这种非核糖体多肽是由微囊藻毒素合成酶产生的，微囊藻毒素合成酶是一种独特的酶复合体，由多肽合成酶、聚酮合成酶和整合的辅酶组成。我们已经鉴定并鉴定了编码这种酶的广泛的基因簇。该项目描述了微囊藻毒素合成酶中特定酶活性的生化特征，以及它们如何确定多种形式的微囊藻毒素的最终结构和毒性。该酶复合体与其底物氨基酸之间的相互作用将为该酶及类似天然产物的基因工程提供信息。

Biosynthesis of nonribosomal peptide toxins in cyanobacteria: A functional characterisation of microcystin synthetase. Microcystins are potent toxins and tumour promoters produced by cyanobacteria associated with blue-green algal blooms. This non-ribosomal peptide is produced by microcystin synthetase, a unique enzyme complex comprised of peptide synthetases, polyketide synthases, and integrated accessory enzymes. We have identified and characterised the extensive gene cluster encoding this enzyme. This project describes the biochemical characterisation of specific enzyme activities within microcystin synthetase and how they determine the final structure and toxicity of the many forms of microcystin. Interactions between this enzyme complex and its substrate amino acids will provide information for the genetic engineering of this and similar natural products.