Components to support a Spinning Disk Confocal Microscope

约克大学的一些研究人员使用显微镜等技术研究细胞。我们对细胞内的事物如何工作特别感兴趣，传统上我们使用显微镜研究细胞的不同部分和细胞中的不同活动，而显微镜只能观察到已经固定的细胞。固定细胞是指突然停止在其轨道上的细胞(固定的)，以便维持细胞的结构和布局，但它们是死的，其中没有任何功能。现在有了更先进的显微镜，可以让我们观察活细胞，并实时记录细胞功能的活动。这显然是一种研究活细胞的有效方法，可以了解细胞的不同部分在不同条件下的表现，或许还包括不同类型的处理。在这项申请中，我们请求支持我们已经获得的一种新显微镜的一些组件，这将允许大量研究人员使用旋转圆盘共聚焦显微镜上的活细胞成像来研究细胞生物学的各个方面。

A number of researchers at York University study cells using techniques such as microscopy. We are particularly interested in how things work inside cells and traditionally have studied different parts of cells and different activities in cells using microscopes that can only look at cells that have been "fixed". Fixed cells are cells that have been suddenly stopped in their tracks (fixed) so that the structure and layout of the cells are maintained but they are dead and nothing is functioning in them. There are now more advanced microscopes that will allow us to look at living cells and to record activities of cellular functions in real time. This is obviously a powerful approach to study living cells and see how various parts of the cells are behaving in different conditions and perhaps with different types of treatments. In this application, we are requesting support for some components of a new microscope that we have acquired which will allow a large number of researchers to investigate various aspects of cell biology, using live cell imaging on a spinning disk confocal microscope.