Analysis of dynamics and protein turnover in integrin-mediated adhesion in drosphilia muscles.

多细胞生物体中的细胞排列成非常多样化的结构阵列。我的主要科学兴趣是确定细胞如何聚集在一起形成复杂的结构，然后，一旦形成，这些结构是如何维持的。我感兴趣的是决定细胞如何相互作用以及与环境相互作用的基本细胞机制。我特别感兴趣的是细胞粘附在动物发育过程中是如何被利用的。我使用一种类型的粘附，细胞粘附到细胞外基质，作为一个模型来解决有关粘附在发育中的作用的基本问题。整联蛋白家族的粘附受体是粘附复合物的核心组分，其对于细胞附着至细胞外基质是重要的。整联蛋白粘附的拆卸和组装受到仔细调节，但目前尚不清楚整联蛋白周转的精确调节以何种方式有助于动物发育。我已经表明，这是可能的，以可视化的装配和动态的整合素粘附复合物，使用实时成像标记的整合素相关蛋白在胚胎肌肉。我已经表明，在发展过程中，整合素粘附的动态变化。许多与整联蛋白粘附相关的蛋白质是GFP标记的，并且在细胞培养物中改变整联蛋白粘附动力学的整联蛋白相关蛋白质的修饰现在可在蝇中获得。我将进行一个系统的调查营业额的整合素粘附动力学内的苍蝇。

Cells in multicellular organisms are arranged in a remarkably diverse array of structures. My main scientific interest is in determining how cells come together to form complex structures and then, once formed, how such structures are maintained. I am interested in the basic cellular mechanisms that determine how cells interact with each other and with their environment. In particular I am interested in how cell adhesion is utilized during animal development. I use one type of adhesion, cell adhesion to the extracellular matrix, as a model to address fundamental question about the role of adhesion in development. Adhesion receptors of the integrin family are the core components of an adhesion complex that is important for the attachment of cells to the extracellular matrix. The disassembly and assembly of integrin adhesions is carefully regulated but it is currently not known in what ways the precise regulation of the turnover of integrin contributes to animal development. I have shown that it is possible to visualize the assembly and dynamics of integrin adhesion complexes using live imaging of tagged integrin associated proteins in embryonic muscles. I have shown that the dynamics of integrin adhesion vary during development. Many of the proteins associated with integrin adhesion are GFP-tagged and modifications in integrin-associated proteins that alter the dynamics of integrin adhesions in cell culture are now available in flies. I will carry out a systematic survey of turnover of integrin adhesion dynamics within the fly.