Which variants of influenza viruses commonly circulate in Ontario swine?

这项拟议工作的目标是确定哪些类型的流感病毒在安大略省的猪身上传播。这将通过对具有代表性的流感病毒分离株进行全序列分析来实现。通过对本研究中流感病毒检测呈阳性的样品进行血凝素(HA)流感病毒糖蛋白基因片段的系统发育分析，确定什么是具有代表性的流感病毒分离株，并通过在鸡胚中进行病毒学试验进行评估。这项研究的样本将通过两种抽样机制中的一种进行收集：(I)定期走访猪群；(Ii)在养猪场调查流感样疾病。现场样本(即鼻拭子)将由人口医学系(Guelph大学)的现场工作人员或畜群兽医提交给研究实验室。为了收集样本，将使用标准的方案。这些信息将对疫苗制造商生产最新的猪流感疫苗非常重要。此外，重要的是，生产商要在知情的情况下决定选择哪些疫苗的毒株与安大略省流行的毒株最相似。这项研究中的信息也可以用来追踪安大略省猪群中优势菌株的长期趋势。

The objective of the proposed work is to determine which types of influenza viruses circulate in Ontario swine. This will be achieved by full sequence analysis of representative influenza virus isolates. What is a representative influenza virus isolate will be determined by doing phylogenetic analysis of the gene segments encoding the hemagglutinin (HA) influenza virus glycoproteins of samples that tested positive for influenza virus in this study, as evaluated by virological test in embryonated chicken eggs. Samples for the study will be collected through one of the two sampling mechanisms: (i) regular herd visits, (ii) investigation of influenza-like disease on swine farms. Field samples (ie, nasal swabs) will be submitted to the research laboratory either by a field crew from the Department of Population Medicine (University of Guelph), or by herd veterinarians. To collect samples, a standard protocol will be used. This information will be important for vaccine manufacturers in order to produce the most updated vaccine for influenza in swine. In addition, it will be important for producers to make informed decisions about the choice of vaccines that would have the strains most similar to strains that circulate in Ontario. The information in this study could also be used for tracing long-term trends of dominant strains in the Ontario swine population.