Crop protection against seedling root rot using bacterial biocontrol systems

苗期病害复合体是引起根枯病和根腐病的主要原因，可导致部分或全部 植物林的损失。虽然传统的广谱杀菌剂已被证明对 保护加拿大头号经济作物，种植者正在寻找替代的作物保护方法 使用细菌生物控制系统。改善苗木的建立是加拿大西部的主要驱动力 像油菜这样的作物。因此，找到新的和创新的方法来保护这种宝贵的加拿大作物是一种 油菜籽种植者的当务之急。拟议的Engage项目将支持初步研究和 在马尼托巴大学的产业界和科学家之间发展协同效应，以调查 建立了油菜幼苗抗真菌的细菌生防体系。 贝尔蒙特实验室将与斯托勒企业一起调查PA23的行动模式， 生防细菌系统，使用下一代RNA测序和全面的 揭示负责作物保护的基因和基因调控网络的生物信息学方法 抗土壤传播的真菌病原体。我们的工作是假设驱动的，将培养至少一名高素质 在这项技术开发方面拥有深厚专业知识的兼职技术人员。这项工作的影响是 广泛应用于加拿大和全球种植的农作物，并将通过 基于发现的科学。PA23配方的优化及其遗传和分子模式的研究 行动将增加这一产品的价值，并改善加拿大的农业经济。这项工作既是 理论上与翻译应用程序，并将导致开发新的工具，很可能 改变我们对自然发生的生物应用的理解，在加拿大最大的 重要的经济作物。

Seedling disease complex is the leading cause of root blight and root rot and can lead to the partial or complete loss of the plant stand. While conventional broad spectrum fungicides have been shown to be effective in protecting Canada's number one cash crop, growers are looking for alternative methods of crop protection using bacterial biocontrol systems. Improved seedling establishment is a major driver for western Canadian crops like canola. Thus, finding new and innovative ways to protect this valuable Canadian crop is an immediate priority for canola growers. The proposed Engage project will support preliminary research and develop synergies between industry and scientists at the University of Manitoba to investigate the effect of an established bacterial biocontrol system in the protection of canola seedlings against fungal pathogens. Together, the Belmonte laboratory with Stoller Enterprises will be investigating the mode of action of PA23, a biocontrol bacteria system, using a combination of next generation RNA sequencing and comprehensive bioinformatics approaches to uncover the genes and gene regulatory networks responsible for crop protection against soil borne fungal pathogens. Our work is hypothesis driven and will train at least one high qualified part time technician with deep expertise in the development of this technology. The impact of this work has broad agricultural application to crops grown in Canada and around the globe and will drive innovation through discovery based science. Optimization of PA23 formulations and understanding its genetic and molecular mode of action will add value to this product and improve Canada's agricultural economy. This work is both theoretical with translational applications and will lead to the development of novel tools that will likely change our understanding of naturally occurring biological applications to the field in one of Canada's most economically important crops.