Delimitation of protein function & interaction networks through peptide arrays

在将基因组发现转化为药物的过程中，一个关键的瓶颈是，基因突变如何导致蛋白质功能和伴随的蛋白质-蛋白质相互作用(PPI)或调控网络的异常变化并不明显，也不容易推断。要克服这一限制，关键是要设计出简便和强大的策略来解码驱动疾病发生和发展的功能蛋白质组。多肽阵列是研究蛋白质在健康和疾病中的功能和连接性的有效方法。在纤维素膜上直接合成数以千计的多肽的能力给蛋白质组学带来了革命性的变化。李博士和共同申请者Litchfied、O‘Donoghue和Shilton博士一直在进行蛋白质组学和化学生物学的尖端研究，这些研究在很大程度上依赖于多肽阵列和可溶性多肽。这群多产和创新的科学家必须能够接触到最先进的多肽和多肽阵列合成仪器，以便他们在蛋白质组学和相关领域进行尖端研究。

A critical bottleneck in the path of translating a genomic discovery to medicine is that it is not apparent or easily deducible how gene mutations result in aberrant changes in protein function and the accompanying protein-protein interaction (PPI) or regulatory network. To overcome this limitation, it is critical to devise facile and robust strategies to decode the functional proteome that drives disease initiation and progression. Peptide array is a powerful method by which to interrogate the function and connectivity of proteins in health and disease. The ability to synthesize thousands of peptides directly on cellulose membranes has brought transformative changes in proteomics. Dr. Li and the co-applicants, Drs. Litchfied, O’Donoghue and Shilton, have been conducting cutting edge research in proteomics and chemical biology which is dependent, in large part, on peptide arrays and soluble peptides. It is essential that this group of productive and innovative scientists to have access to the state-of-the-art instrumentation in peptide and peptide array synthesis in order for them to conduct cutting edge research in proteomics and related fields.