Gas-Chromatography with Atmospheric Pressure Chemical Ionization interface (GC-APCI) for Flavouromics Research

所要求的设备是一个气相色谱（GC）系统，该系统将与现有的四极杆飞行时间（QTOF）质谱仪集成，以高质量精度识别挥发性有机化合物（VOC）。这将建立一个世界级的系统，用于确定和识别非挥发性、挥发性和半挥发性化合物，并大大推进申请人的研究和培训。使用该系统可以实现快速分离，半挥发性和挥发性化合物的分离效率和分辨率均非常高，质量精度为2 ppm。申请人必须进行与各种食品和作物中VOCs形成及其与其他食品成分潜在相互作用相关的跨学科研究合作。此外，所要求的仪器将允许识别在我们以前的研究中显示出有益功能的食品中的生物活性化合物。申请人是经验丰富的NSERC资助的研究人员，并建立了合作项目，共同持有的资金证明。当添加到现有的完全食品级，感官研究实验室和液相色谱-QTOF能力，所要求的系统将放置马尼托巴大学和申请人的研究能力在加拿大的首要位置，包括风味和生物活性化合物的鉴定研究。该设备将显著影响申请人在2个不同部门之间的现有合作，并将提供一个独特的平台，可供目前的9名研究生和3名技术人员使用，并在未来5年内增加12名研究生和4名本科生。

The requested equipment is a Gas Chromatography (GC) system that will be integrated with an existing Quadrupole Time of Flight (QTOF) Mass Spectrometer to identify volatile organic compounds (VOCs) with a high mass accuracy. This will create a world class system for determination and identification of non-volatiles, volatiles and semi-volatile compounds and substantially advance the applicants' research and training. Fast separations with exceptional efficiency and resolution for semi-volatiles and volatile compounds with a 2 ppm mass accuracy will be achieved using this system. It is necessary for the applicants' interdisciplinary research collaboration related to VOCs formation in various foods and crops and their potential interactions with other food components. In addition, the requested instrument will permit the identification of bioactive compounds in foods that have shown beneficial functionalities in our previous studies. The applicants are experienced NSERC-funded researchers and have established collaborative projects as demonstrated by jointly held funding. When added to existing fully food-grade, sensory research laboratories and liquid chromatography-QTOF capabilities, the requested system will place the University of Manitoba and the research capabilities of the applicants at the premier position in Canada for studies comprising flavouromics and identification of bioactive compounds. This equipment will significantly impact the existing collaboration among applicants in 2 different departments and will provide a unique platform which can be used by the current 9 graduate students and 3 technicians, and within the next 5 years 12 more graduate and 4 undergraduate students across the combined NSERC programs.