Impact of reactive oxygen species on surface localization of membrane proteins

细胞通过驻留在细胞表面的蛋白质相互作用，并与周围环境相互作用。位于细胞表面的蛋白质在形成将细胞连接在一起的连接方面发挥着作用，对我们身体产生的化学物质和激素做出反应，对药物和许多其他过程做出反应。这些蛋白质不是固定在一个位置，但在许多情况下，它们既可以在细胞表膜内移动，允许与其他蛋白质进行复杂的相互作用，也可以进出表膜，从而控制它们的可用性。这种运动的一部分是由小窝的形成控制的。小凹是细胞膜上的内陷，可以作为“信号域”，或者可以发芽从表面移除蛋白质。小窝蛋白-1是一种与小窝相关的支架蛋白，可被过氧化氢等活性氧物种激活。细胞中活性氧的主要来源之一是一种名为NADPH氧化酶或NOX酶的酶家族。我们的目标是确定NADPH氧化酶衍生的ROS、小窝蛋白-1的磷酸化与细胞表面蛋白的激活、运动的关系。

Cells interact with one other and with their surroundings through proteins that reside at the cell surface. Proteins located at the surface of the cell play a role in forming connections that hold cells together, responding to the chemicals and hormones our bodies produce, responding to drugs and in many other processes. These proteins do not remain fixed in one location, but in many cases, they move around both within the cell surface membrane allowing complex interactions with other proteins, as well as to and from the surface membrane allowing control over their availability. Some of this movement is controlled by the formation of caveolae. Caveolae are invaginations in the cell membrane that can act as “signaling domains” or can bud off to remove proteins from the surface. Caveolin-1 is a caveolae-associated scaffolding protein that can be activated by reactive oxygen species (ROS) such as hydrogen peroxide. One of the main sources of reactive oxygen species in cells is a family of enzymes called NADPH oxidases, or NOX enzymes. We aim to define the relationship between NADPH oxidase derived ROS, caveolin-1 phosphorylation and activation, movement of cell surface proteins.