Novel platforms for detection of Mycobacterium avium subsp. paratuberculosis infection in dairy cattle.

约翰氏病(JD)是一种由禽分枝杆菌亚种引起的反刍动物慢性肠炎，使人衰弱。副结核(MAP)是乳品行业的主要健康问题之一，对经济造成重大影响。目前的诊断测试表现不佳，特别是在感染的早期阶段，严重阻碍了预防和控制计划。他们未能在感染动物传播疾病之前可靠地检测到它们。目前用于抗体(Ab)检测的ELISA具有粗制的抗原(Ag)混合物，不适合早期检测。粪便脱落和抗体反应相关性较差，但联合检测这两种方法可提高检测灵敏度。

Johne’s disease (JD), a debilitating chronic enteritis in ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map), is one of the main health issues in the dairy industry, resulting in significant economical impact. Prevention and control programs are severely hampered by the poor performance of current diagnostic tests, especially in the early stages of the infection. They fail to reliably detect infected animals before they can spread the disease. Current ELISAs for antibody (Ab) testing have crude antigen (Ag) mixtures that are not optimized for early detection. Fecal shedding and Ab responses correlate poorly, but combined testing for these increases testing sensitivity.