Roles of Malat1 in response to hypoxia

我们的团队之前通过nserc资助的项目发现了长链非编码RNA Malat1，使用基因表达的平行修饰来筛选对脂质代谢至关重要的组织（即肝脏和脂肪）中随衰老和饮食调节的基因。我们最近证明，低氧条件下通过刺激AMPK/HIF-1？轴。基于这些新发现，我们建议研究Malat1在低氧条件下或通过激活脂多糖（LPS）诱导的炎症反应中对细胞和生理适应的调节中的作用。使用体外和体内模型，我们将：

Our team previously uncovered the long non-coding RNA Malat1 through NSERC-funded programs using parallel modifications in gene expression to screen for genes modulated upon aging and diet in tissues critical for lipid metabolism, namely liver and adipose. We recently demonstrated that low oxygen conditions robustly increase Malat1 gene expression through the stimulation of an AMPK/HIF-1? axis. Based on these novel findings, we propose to study the contribution of Malat1 in the modulation of cellular and physiological adaptations that take place in response to hypoxia, either triggered in low oxygen conditions, or through activation of lipopolysaccharide (LPS)-induced inflammation. Using both in vitro and in vivo models, we will: