New Dimensions and Domains for Fluorescence Fluctuation Imaging Methods

在过去的十年里，光学显微镜领域发生了一场革命，2014年诺贝尔化学奖授予Betzig，Moerner和Hell，以表彰他们对超分辨荧光显微镜的发展。这场技术复兴见证了超分辨率荧光显微镜和单面照明显微镜(SPIM)的巨大进步，SPIM也通常被称为光片显微镜。就像17世纪光学显微镜出现时一样，这些新的技术飞跃为活细胞和生物的生物医学研究科学提供了巨大的希望。然而，许多形式的超分辨率显微镜是基于迭代分子定位的，这导致了动力学瓶颈，其中牺牲了时间分辨率来获得空间分辨率。

In the past decade there has been a revolution in light microscopy recognized with the awarding of the 2014 Chemistry Nobel Prize to Betzig, Moerner and Hell for the development of super resolved fluorescence microscopy. This technical renaissance has witnessed tremendous advances in both super-resolution fluorescence microscopy and in single plane illumination microscopy (SPIM) also commonly known as light sheet microscopy. As was the case at the advent of light microscopy in the 17th century, these new technological leaps offer great promise for biomedical research science on living cells and organisms. However, many forms of super-resolution microscopy are based on iterative molecular localization leading to a kinetic bottleneck where temporal resolution is sacrificed to gain spatial resolution.