Extraction of rosavin, rosin and rosarin from Rhodiola rosea roots using supercritical CO2

红景天是一种草本植物，发现于北极地区和阿尔伯塔省，传统上用于药用。各种研究已经证明了红景天在几个健康领域的生物活性，包括压力和癌症等。在治疗效果方面的兴趣是它的保存化合物。采用不同的溶剂萃取方法从红景天根中分离出了迷迭香素。然而，利用这些现有的提取物，所得到的番石榴的数量和纯度在质量和产量方面仍然是一个重大的挑战。此外，人们对这种生物活性复合物的玫瑰苷和玫瑰苷成分的提取知之甚少，这些成分统称为玫瑰苷。该项目的主要目的是利用超临界CO2萃取技术，一种环保的“绿色”技术，从圣弗朗西斯药草农场公司提供的红景天根中提取玫瑰香、松香和玫瑰香。超临界萃取采用二氧化碳作为溶剂，可以很容易地将溶剂从提取的玫瑰香、松香和玫瑰香中分离出来，从而得到易于表征的产品。超临界萃取的结果将在收率和产品质量方面与常规溶剂萃取进行比较。高产量的纯rosavin、松香和rosarin将允许它们作为加拿大生产的天然保健品的成分进一步商业化，并将加强加拿大的农业部门。玫瑰香、松香和玫瑰香的成功提取和商业化也具有社会效益，可能为全世界受这些健康问题影响的大部分女性和男性提供负担得起的治疗潮热、压力和抑郁的方法。

Rhodiola rosea is an herbaceous plant found in the Arctic region and Alberta that has traditionally been usedfor medicinal purposes. Various studies have demonstrated the bioactivity of Rhodiola rosea in several areas ofhealth, including stress and cancer, among others. Of interest in terms of therapeutic effectiveness are itsrosavin compounds. Different solvent extraction methodologies have been used to isolate rosavin fromRhodiola rosea roots. However, with these existing extracts the amount and purity of the resulting rosavin interms of both quality and yield remain a significant challenge. Also, little is known about the extraction of therosin and rosarin components of this bioactive complex collectively called rosavins. The main objective of thisproject is to use supercritical CO2 extraction, an environmentally-friendly "green" technology, to extractrosavins, i.e. rosavin, rosin and rosarin, from Rhodiola rosea roots provided by St. Francis Herb Farm Inc.Supercritical extraction using carbon dioxide as the solvent allows easy separation of the solvent from theextracted rosavin, rosin, and rosarin, leaving a product that is easy to characterize. Results from supercriticalextraction will be compared to conventional solvent extraction in terms of both yield and product quality. Ahigh yield of pure rosavin, rosin, and rosarin will allow their further commercialization as ingredients of naturalhealth products produced in Canada and will strengthen Canada's agricultural sector. The successful extractionand commercialization of rosavin, rosin, and rosarin also has the social benefit of potentially providing anaffordable treatment for hot flashes, stress, and depression to a large segment of women and men affected bythese health issues worldwide.