Comprehensive assessment of mitochondrial bioenergetics; enabling simultaneous measurements in controlled oxygen environments

这是一个尖端的线粒体生物能量学系统的要求，可以同时确定线粒体功能的几个方面。细胞在分解食物产生能量（ATP）的同时消耗氧气。评估线粒体功能的历史黄金标准反映了氧气消耗率的确定，这种方法已成为我们研究计划的基石。然而，这种方法的一个主要限制是不能确定ATP的产生速率，而ATP的产生速率是氧化磷酸化的最终目标。所要求的设备将能够同时评估氧气消耗和ATP产生，消除这种方法上的限制，同时还能够设计更多样化和生物学相关的机制研究。

This is a request for a cutting-edge mitochondrial bioenergetics system that can simultaneously determine several aspects of mitochondrial function. Cells consume oxygen while breaking down food to produce energy (ATP). The historical gold-standard for assessing mitochondrial function reflects determining rates of oxygen consumption and this methodology has become the corner-stone of our research programs. However, a major limitation of this approach is that rates of ATP production are not determined, which is the ultimate goal of oxidative phosphorylation. The requested piece of equipment will enable simultaneous assessments of oxygen consumption and ATP production, negating this methodological limitation, while also enabling more diverse and biologically relevant mechanistic studies to be designed.