Refrigerated microcentrifuge for radioisotope samples in life sciences research

*我们建议为麦吉尔大学寄生虫学研究所购买一台冷冻微型离心机。该仪器将是专门用于分析方法和生物应用的放射性同位素工作的新设施的一部分。它将设在寄生虫学研究所，以便方便和安全地进入，其中将包含涉及各种生物样本的放射性同位素标记的实验。它将支持寄生虫学研究所的几个研究小组，他们将使用放射性同位素进行需要控制恒温的敏感分析方法。此外，每年有50多名研究生受训于寄生虫学研究所管理的生物技术领域的论文和非论文研究生课程，他们可以从这一设施和设备中受益。因此，预计将由R.Salavati博士管理和指导大量使用。冷冻的微型离心机将用于处理和制备大分子，包括核酸(RNA和DNA)、蛋白质和由放射性同位素(即32P、35S、3H)标记的小化合物，以提高灵敏度，并随后用于各种酶和结合分析。在这类分析的样品制备过程中，受控温度对于保持大分子和小化合物的完整性以保持充分的活性至关重要。因此，该仪器的可用性将是支持研究计划(S)的关键，并极大地促进研究能力和多学科培训环境。

***We propose to purchase a refrigerated microcentrifuge for the Institute of Parasitology at McGill University. The instrument will be part of a new facility dedicated to radioisotope work for analytical methods and biological applications. It will be located at the Institute of Parasitology for convenient and safe access that will contain the experiments involving radioisotope labeling of various biological samples. It will support several research groups at the Institute of Parasitology who will use radioisotopes for sensitive analytical methods that will require controlled constant temperature. Also, more than 50 graduate student trainees per year in both thesis and non-thesis graduate programs administered by the Institute of Parasitology in the field of biotechnology can benefit from this facility and equipment. Therefore, significant usage is anticipated that will be managed and directed by Dr. R. Salavati. The refrigerated microcentrifuge will be used to process and prepare macromolecules including nucleic acids (RNA and DNA), proteins, and small chemical compounds that are labeled by a radioisotope (i.e., 32 P, 35S, 3H,) for increased sensitivity and subsequent use in a variety of enzymatic and binding assays. The controlled temperature during sample preparation for such assays is essential in maintaining the integrity of macromolecules as well as small chemical compounds to remain fully active. Therefore, the availability of this instrument will be essential to support the research program(s) and significantly facilitate both research abilities and a multidisciplinary training environment.