Enhancing the efficacy of Pseudomonas entomophila 23S, a PGPR for biocontrol of bacterial canker disease in tomato ******

细菌性溃疡病，由Clavibacter michiganensis subsp. michiganensis（Cmm）是番茄上最严重的病害之一。该病害已造成大量作物损失，并成为包括加拿大在内的世界许多地区田间和温室番茄生产的严重问题。现有的控制策略，如种子处理和化学防治，在控制这种疾病方面只显示出有限的效果。因此，迫切需要有效的控制策略。麦吉尔大学的史密斯实验室已经发现了几种对Cmm表现出拮抗活性的菌株，并且分离出由这些菌株之一假单胞菌23 S产生的抗Cmm化合物。鉴于病害的严重性，这种抗Cmm化合物以及产生的菌株作为番茄细菌性溃疡病的一种新的有效控制策略具有很大的潜力。Lallemand一直对开发一种控制CMM的产品感兴趣，并一直在寻找潜在的方法。凭借Lallemand在菌株生产、扩大规模、配方和产品开发方面的专业知识，拟议工作的成功将使我们能够充分了解基于假单胞菌23 S的技术的潜力，并最终在加拿大全面部署。我们拟对假单胞菌23 S产生的抗Cmm化合物进行研究，并确定最佳生产方法。该项目将包括阐明抗Cmm化合物的最低抑制浓度，研究该化合物在不同孵育阶段的浓度，并检查Cmm对假单胞菌23 S产生的抗Cmm化合物的影响。这项工作将提高加拿大番茄生产商的可持续性和经济效益，并使他们在北美番茄生产市场上进一步领先。这项工作将提供对新发现的由假单胞菌23 S产生的抗Cmm化合物及其最佳生产方法的更深入了解，从而导致Lallemand和麦吉尔大学之间更广泛的合作;这将允许进一步利用生物制剂和开发农业新产品。

Bacterial canker disease, caused by Clavibacter michiganensis subsp. michiganensis (Cmm) is one of the most serious diseases of tomato. The disease has caused substantial crop losses and become a serious concern for both field and greenhouse tomato production in many parts of the world, including Canada. Existing control strategies, such as seed treatment and chemical control, have shown only limited efficacy in controlling this disease. Thus, effective control strategies are urgently required. The Smith laboratory at McGill University has discovered several strains that show antagonistic activity against Cmm, and an anti-Cmm compound produced by one of these strains, Pseudomonas sp. 23S was isolated. Given the seriousness of the disease damages, this anti-Cmm compound, as well as the producing stain is great potential as a new effective control strategy for tomato bacterial canker. Lallemand has been interested in developing a product to control Cmm and has been looking for potential approaches. With Lallemand's expertise on strain production, scale-up, formulation and product development, success in the proposed work would allow a full effort to completely understand the potential of a technology based on Pseudomonas sp. 23S and eventual full deployment in the Canadian context. We propose to study the anti- Cmm compounds produced by Pseudomonas sp. 23S and to determine the best production method. The project will consist of elucidating the minimum inhibitory concentration of the anti-Cmm compound, studying the concentration of the compound during various incubation phases, and examining the effects of Cmm on the production of anti- Cmm compounds produced by Pseudomonas sp. 23S. This work will improve the sustainability and economics of Canadian tomato producers and give them further leadership in the North American tomato production market. The work will provide a deeper understanding of the newly discovered anti-Cmm compound produced by Pseudomonas sp. 23S and its best production method, leading to a broader collaboration between Lallemand and McGill University; one that will allow further utilization of biologicals and the development of new products in agriculture.