Développement de biocatalyseurs novateurs de laccase et de tyrosinase pour l'élimination des polluants émergents dans les eaux usées

在这项赠款申请中，我们建议开发有效的技术来消除新出现的污染物，包括作为内分泌干扰物的化合物。我们将继续开发聚合酶，特别是包括两种或更多酶的组合。这项拟议的工作将在包括膜生物反应器在内的新型反应器中使用作为Clea凝聚的酶。CLEA最初将是单一酶，如漆酶。膜生物反应器的CLEA将是一种以上酶的CLEA。这些组合将允许处理真实废水，否则温度、pH和其他化合物的存在将使酶无效或至少迫使它们在不理想的条件下运行。治疗的目标化合物将包括双酚A、三氯生和壬基酚。膜生物反应器应能在高流速、低污染物浓度下运行，且酶损失很小。该项目将使用酶对酚类化合物进行有效的处理。此外，这种治疗将是可行的，因为有可能以合理的费用进行这种治疗。

In this grant application, we propose to develop effective techniques to eliminate emerging pollutants including compounds which are endocrine disruptors. We will continue the development of aggregated enzymes especially combi-CLEAs which include two or more enzymes. The work proposed will use enzymes agglomerated as CLEA's in novel reactors which will include membrane bioreactors. The CLEAs will initially be of single enzymes such as laccase. The CLEAs for the membrane bioreactor will be CLEA's of more than one enzyme. These combi-CLEAs will allow the treatments of real wastewater where temperature, pH, and the presence of other compounds would otherwise make the enzymes either ineffective or at least force them to operate in less than optimal conditions. Target compounds for treatment will include bisphenol A, triclosan and nonylphenol. The membrane bioreactor should be able to operate at high flow rates, low pollutant concentrations with little loss of enzymes. This project will have as its outcome effective treatment for phenolic compounds using enzymes. Moreover the treatment will be practicable in the sense that it will be possible to undertake such treatment at a reasonable cost.