High throughput equipment for generating next generation sequencing libraries

下一代测序(NGS)使研究人员能够快速表征基因组和遗传多样性、基因表达和表观遗传修饰，甚至是在宿主物种和整个生态系统中发现的相互作用的生物的复杂组合，从而使生物学发生了革命性的变化。在这里，我们需要一套高通量设备，在核酸提取之前进行NGS组织裂解、超声产生适当大小的DNA或RNA片段以构建序列文库以及核酸和测序文库的质量控制评估中的三个重要步骤。迫切需要这种设备来为包括几名早期职业研究人员在内的越来越多的研究人员在NGS中创造能力，以培训一大批不同的HQP，了解按需研究技术(从而提供相当多的就业机会)，并极大地提高七名教师研究计划的成本和时间效率。

Next generation sequencing (NGS) has revolutionized biology by allowing researchers to rapidly characterize genomes and genetic diversity, gene expression and epigenetic modifications, and even the complex assemblages of interacting organisms found within host species and entire ecosystems. Here, we request a suite of high-throughput equipment needed to undertake three important steps in NGS tissue lysis prior to nucleic acid extraction, sonication to generate appropriately sized DNA or RNA fragments for constructing sequence libraries, and quality control assessment of nucleic acids and sequencing libraries. This equipment is urgently needed to create capacity in NGS for a growing number of researchers, including several early career researchers, to train a large cohort of diverse HQP on in-demand research techniques (thereby providing considerable employment opportunities), and to vastly increase the cost and time efficiency of the research programs of seven faculty.