Cryopreservation and polyploid breeding strategies for Cannabis sativa

大麻已成为加拿大最重要的经济作物之一，每年为国内生产总值贡献数十亿美元。尽管如此，长期的禁种历史导致了现代研究的普遍缺乏，与其他作物相比，我们对植物生物学和生物技术工具的基本了解存在不足。在育种技术方面尤其如此，那里非常需要遗传保存系统和发展无籽三倍体或更高倍性水平的潜力。初步报告是有希望的，但关于这一方法的影响和潜力仍有许多问题。在这个项目中，我们的目标是通过改进超低温保存方法来解决其中的一些不足，以促进克隆系的长期保存，并评估用于大麻遗传改良的多倍体育种方法。这些项目将与先进的新陈代谢和基因组学工具相结合，以深入了解冷冻保存材料的遗传保真度，并就增加倍性的影响提供详细信息。这些实验将为大麻生物学提供新的见解，并确保随着行业的发展，加拿大大麻行业保持全球竞争力。

Cannabis has become one of the most economically important crops in Canada and it contributed billions of dollars to the GDP each year. Despite this, the long history of prohibition has resulted in a general lack of modern research and in comparison to other crops, there is a deficit in our basic understanding of the plants biology and biotechnological tools. This is particularly true in the case of breeding technologies, where there is great need for genetic preservation systems and potential for the development of seedless triploids or higher ploidy levels. Preliminary reports are promising, but there remains many questions on the implications and potential of this approach. In this project, we aim to address some of these deficiencies by refining cryopreservation methods to facilitate the long term conservation of clonal lines and evaluate polyploid breeding methodologies for genetic improvement in cannabis. These projects will be coupled with advanced metabolomic and genomic tools to gain in depth understandings of the genetic fidelity of cryopreserved material, and detailed information on the impacts of increased ploidy. These experiments will provide new insights into cannabis biology and ensure that the Canadian Cannabis industry remains globally competitive as the industry expands.