Development and functional characterization of a volumetric fluorescence lifetime videorate microscope

为了获得体内细胞网络功能的综合视角，成像应用变得越来越复杂和多样化，需要多种模式的结合。今天，荧光成像是研究动态生物过程的一种重要方式。它是定量测量分子相互作用和离子浓度的金标准。然而，其在体内的应用目前受到商用光学技术的限制。传统的荧光显微镜系统受到光学切片和低成像速度的限制。最近，贝塞尔型体积成像已经证明了它在快速体内成像方面的有用性，并允许对活体生物样本进行功能研究。通过提供独特和适应的技术产品，Bliq Photonics为客户提供最先进的解决方案。视频速率体积显微镜与荧光寿命成像的结合将使在体内宏观尺度上观察脑结构的分子相互作用民主化。此次合作产生的产品以及通过该项目开发的神经科学和细胞生物学的新应用将为Bliq Photonics提供主要的商业资产，并将使其能够扩大其产品和市场份额。

To obtain an integrative perspective of cell network functions in vivo, imaging applications are becoming increasingly complex and diverse, requiring the combination of several modalities. Today, fluorescence imaging is an essential modality to study dynamic biological processes. It is the gold standard for quantitatively measuring molecular interactions and ionic concentrations. However, its in vivo application is currently limited by commercially available optical technologies. Conventional fluorescence microscopy systems are limited by the optical sectioning and the low imaging acquisition speed. Recently, Bessel-type volumetric imaging has demonstrated its usefulness for rapid in vivo imaging and has allowed the functional study of living biological samples. By offering unique and adapted technological products, Bliq Photonics addresses customers looking for state-of-the-art solutions. The combination of a video rate volumetric microscope with fluorescence lifetime imaging will democratize the observation of molecular interactions at the macroscopic scale of brain structures in vivo. The product resulting from this collaboration and the new applications in neuroscience and cell biology developed through this project will provide Bliq Photonics with a major commercial asset and will allow it to expand its offer and market share.