Redox signaling: A key element for the development of new post-harvest conditioning technologies

食用园艺作物是至关重要的，因为它们含有大量的营养物质，而且人们普遍认为食用它们与减少退行性疾病之间存在反比关系。因此，这些产品的供应链在各个阶段都必须得到保障，最后一个阶段是收获后的储存。正确处理水果和蔬菜会给整个生产链带来经济效益，也会让顾客满意。然而，这可以更进一步，通过收获后处理，可以修改农产品的初级和次级代谢。这些改变可以延长农产品的保质期，并导致功能性新鲜农产品的发展。后者包括植物化合物含量增加的新鲜农产品，具有药理学（即免疫学）特征的新鲜农产品，以及具有改善的味道和气味特征的新鲜农产品。以前，我们已经表明，在低剂量下，不同的非生物胁迫似乎有利于保持农产品的质量和可储存性。然而，我们还没有发现导致这些积极变化的早期事件。这就提出了一个问题，即是否有可能通过揭示与钙和电波以及农产品中的氧化还原信号相关的早期事件来开发新的植物产品存储技术。在此背景下，本研究将尝试将氧化还原调控和植物激素信号与氧化和还原胁迫下园艺产品初级和次级代谢的改变联系起来。该研究计划的长期目标是开发量身定制的收获后处理方法，以获得具有增强风味和气味特征，改善植物化学成分和延长储存寿命的功能性商品。这项研究的重要性在于，可以为加拿大生产的园艺产品增加价值，也可以为该国进口的园艺产品增加价值。因此，这可能会导致新鲜未加工工业中一个新部门的开始。在中短期内，与生鲜农产品相关的研究计划框架将包括：(1)乙烯在氧化还原状态和整体代谢中的影响；(2)长期贮藏对氧化还原状态和营养品质的影响；3)还原应激和氧化应激对初级和次级代谢的改变；4)植物激素在细菌源的新型挥发性杀菌剂引发ROS生成中的作用。这些研究将使用西红柿、haskaps、野生蓝莓和草莓进行。因此，提出了一个概念图的发展，以一种机械的方式解释收获后氧化还原信号的滚动，以提高新鲜农产品的质量。

The intake of horticultural crops is of utmost importance due to the amount of nutrients they contain, as well as the generally accepted inverse relationship between their consumption and the decrease in degenerative diseases. Thus, the supply chain of these products must be secured in all its stages, the last one being post-harvest storage. Proper handling of fruits and vegetables results in economic benefits for the entire production chain, as well as customer satisfaction. However, this can be taken a step further, and through post-harvest treatments the primary and secondary metabolism of produce can be modified. These alterations can increase the shelf life of produce and lead to the development of functional fresh produce. The latter include fresh produce with an increased amount of phytochemical compounds, fresh produce with pharmacological (i.e., immunological) character, as well as fresh produce with improved taste and odor characteristics. Previously, we have shown that different abiotic stresses appear to be beneficial in maintaining the quality and storability of produce at low doses. However, we haven't discerned the early events that cause these positive changes. This raises the question of whether it is possible to develop new storage technologies for plant products by unveiling early events related to the calcium and electrical waves, as well as redox signaling in produce. In this context, this research will attempt to relate, redox regulated and phytohormone signaling to modifications of primary and secondary metabolism in horticultural products by oxidative and reductive stresses. The long-term objective of this research program is to develop tailored post-harvest treatments to achieve functional commodities, either with enhanced flavor and odor characteristics, improved phytochemical composition and longer storage life. The importance of this research is that added value can be given to the horticultural products produced in Canada, but also to those that the country imports. Therefore, this could lead to the start of a new sector in the fresh unprocessed industry. In the short and medium term, the framework of the research program related to fresh produce will include: (1) the influence of ethylene in the redox state and overall metabolism; (2) the effect of long-term storage on redox status and nutritional quality; 3) the modification of primary and secondary metabolism due to reducing and oxidative stresses; and 4) the role of phytohormones in the production of ROS derived from the priming action of novel volatile fungicides of bacterial origin. The studies will be carried out using tomatoes, haskaps, wild blueberries and strawberries. Thus, the development of a conceptual map is proposed to explain in a mechanistic way the roll of redox signaling in post-harvest to improve the quality of fresh produce.